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Literature summary for 1.17.1.9 extracted from

  • Zheng, J.; Yang, T.; Zhou, J.; Xu, M.; Zhang, X.; Rao, Z.
    Elimination of a free cysteine by creation of a disulfide bond increases the activity and stability of Candida boidinii formate dehydrogenase (2017), Appl. Environ. Microbiol., 83, e02624-16.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells [Candida] boidinii

Protein Variants

Protein Variants Comment Organism
A10C there are no significant changes in the optimum temperature and pH between variant and wild type enzyme but the mutant shows a significant increase in copper ion resistance and acid resistance, a 6.7fold increase in half-life at 60°C, and a 1.4fold increase in catalytic efficiency compared with the wild type [Candida] boidinii
A10C/I239C there are no significant changes in the optimum temperature and pH between variant and wild type enzyme [Candida] boidinii
I239C there are no significant changes in the optimum temperature and pH between variant and wild type enzyme [Candida] boidinii

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ the wild type enzyme is completely inactivated by 5 mM Cu2+ [Candida] boidinii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0536
-
 NAD+ wild type enzyme, at 30°C and pH 7.0 [Candida] boidinii
0.0742
-
 NAD+ mutant enzyme A10C, at 30°C and pH 7.0 [Candida] boidinii
0.1191
-
 NAD+ mutant enzyme A10C/I239C, at 30°C and pH 7.0 [Candida] boidinii
0.1322
-
 NAD+ mutant enzyme I239C, at 30°C and pH 7.0 [Candida] boidinii
5.2
-
 formate mutant enzyme I239C, at 30°C and pH 7.0 [Candida] boidinii
5.8
-
 formate mutant enzyme A10C/I239C, at 30°C and pH 7.0 [Candida] boidinii
7.3
-
 formate wild type enzyme, at 30°C and pH 7.0 [Candida] boidinii
8.2
-
 formate mutant enzyme A10C, at 30°C and pH 7.0 [Candida] boidinii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formate + NAD+ [Candida] boidinii
-
 CO2 + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
[Candida] boidinii O13437
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography [Candida] boidinii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
 [Candida] boidinii CO2 + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
FDH
-
 [Candida] boidinii
NAD+-dependent formate dehydrogenase
-
 [Candida] boidinii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 55
-
 [Candida] boidinii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 52 the enzyme remains stable after 20 min at 30-52°C. The half-life temperature is 56.7°C. The enzyme is inactivated after 20 min at 60°C [Candida] boidinii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6
-
 NAD+ mutant enzyme I239C, at 30°C and pH 7.0 [Candida] boidinii
3.3
-
 NAD+ wild type enzyme, at 30°C and pH 7.0 [Candida] boidinii
4.3
-
 NAD+ mutant enzyme A10C/I239C, at 30°C and pH 7.0 [Candida] boidinii
6.2
-
 NAD+ mutant enzyme A10C, at 30°C and pH 7.0 [Candida] boidinii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 9.5
-
 [Candida] boidinii

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 [Candida] boidinii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
20
-
 NAD+ mutant enzyme I239C, at 30°C and pH 7.0 [Candida] boidinii
36
-
 NAD+ mutant enzyme A10C/I239C, at 30°C and pH 7.0 [Candida] boidinii
62
-
 NAD+ wild type enzyme, at 30°C and pH 7.0 [Candida] boidinii
84
-
 NAD+ mutant enzyme A10C, at 30°C and pH 7.0 [Candida] boidinii