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Literature summary for 1.17.1.9 extracted from

  • Alekseeva, A.A.; Kargov, I.S.; Kleimenov, S.Y.; Savin, S.S.; Tishkov, V.I.
    Additivity of the stabilization effect of single amino acid substitutions in triple mutants of recombinant formate dehydrogenase from the soybean Glycine max (2015), Acta Naturae, 7, 55-64.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Codon Plus/pLysS cells Glycine max

Protein Variants

Protein Variants Comment Organism
A267M the mutant shows increased catalytic efficiency compared to the wild type enzyme Glycine max
A267M/I272V the mutant shows reduced catalytic efficiency compared to the wild type enzyme Glycine max
A267M/I272V/F290D the mutant shows reduced catalytic efficiency compared to the wild type enzyme Glycine max
A267M/I272V/F290N the mutant shows reduced catalytic efficiency compared to the wild type enzyme Glycine max
A267M/I272V/F290S the mutant shows reduced catalytic efficiency compared to the wild type enzyme Glycine max
F290D the mutant shows reduced catalytic efficiency compared to the wild type enzyme Glycine max
F290N the mutant shows reduced catalytic efficiency compared to the wild type enzyme Glycine max
F290S the mutant shows reduced catalytic efficiency compared to the wild type enzyme Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
 formate wild type enzyme, at pH 8.0 and 37°C Glycine max
2.1
-
 formate mutant enzyme A267M, at pH 8.0 and 37°C Glycine max
2.2
-
 formate mutant enzyme A267M/I272V/F290N, at pH 8.0 and 37°C Glycine max
2.3
-
 formate mutant enzyme A267M/I272V/F290S, at pH 8.0 and 37°C Glycine max
2.4
-
 formate mutant enzyme A267M/I272V, at pH 8.0 and 37°C Glycine max
2.8
-
 formate mutant enzyme A267M/I272V/F290D, at pH 8.0 and 37°C Glycine max
4.1
-
 formate mutant enzyme F290S, at pH 8.0 and 37°C Glycine max
4.5
-
 formate mutant enzyme F290N, at pH 8.0 and 37°C Glycine max
5
-
 formate mutant enzyme F290D, at pH 8.0 and 37°C Glycine max
9.1
-
 NAD+ mutant enzyme F290S, at pH 8.0 and 37°C Glycine max
9.9
-
 NAD+ mutant enzyme A267M, at pH 8.0 and 37°C Glycine max
12.8
-
 NAD+ mutant enzyme F290D, at pH 8.0 and 37°C Glycine max
13.3
-
 NAD+ wild type enzyme, at pH 8.0 and 37°C Glycine max
13.3
-
 NAD+ mutant enzyme A267M/I272V, at pH 8.0 and 37°C Glycine max
14
-
 NAD+ mutant enzyme F290N, at pH 8.0 and 37°C Glycine max
14.1
-
 NAD+ mutant enzyme A267M/I272V/F290N, at pH 8.0 and 37°C Glycine max
16.1
-
 NAD+ mutant enzyme A267M/I272V/F290S, at pH 8.0 and 37°C Glycine max
20.3
-
 NAD+ mutant enzyme A267M/I272V/F290D, at pH 8.0 and 37°C Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, phenyl Sepharose column chromatography, and Sephadex G-25 gel filtration Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
 Glycine max CO2 + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
NAD(P)+.dependent formate dehydrogenase
-
 Glycine max

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
57
-
 melting temperature Glycine max

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2
-
 formate mutant enzyme A267M/I272V, at pH 8.0 and 37°C Glycine max
2.8
-
 formate mutant enzyme F290N, at pH 8.0 and 37°C Glycine max
2.9
-
 formate wild type enzyme, at pH 8.0 and 37°C Glycine max
2.9
-
 formate mutant enzyme A267M/I272V/F290D, at pH 8.0 and 37°C Glycine max
3.2
-
 formate mutant enzyme A267M/I272V/F290N, at pH 8.0 and 37°C Glycine max
3.7
-
 formate mutant enzyme A267M/I272V/F290S, at pH 8.0 and 37°C Glycine max
4.1
-
 formate mutant enzyme F290S, at pH 8.0 and 37°C Glycine max
5
-
 formate mutant enzyme A267M, at pH 8.0 and 37°C Glycine max
5.1
-
 formate mutant enzyme F290D, at pH 8.0 and 37°C Glycine max

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Glycine max

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.62
-
 formate mutant enzyme F290D, at pH 8.0 and 37°C Glycine max
0.92
-
 formate mutant enzyme A267M/I272V, at pH 8.0 and 37°C Glycine max
1
-
 formate mutant enzyme F290S, at pH 8.0 and 37°C Glycine max
1.02
-
 formate mutant enzyme F290N, at pH 8.0 and 37°C Glycine max
1.36
-
 formate mutant enzyme A267M/I272V/F290S, at pH 8.0 and 37°C Glycine max
1.45
-
 formate mutant enzyme A267M/I272V/F290N, at pH 8.0 and 37°C Glycine max
1.61
-
 formate mutant enzyme A267M/I272V/F290D, at pH 8.0 and 37°C Glycine max
1.93
-
 formate wild type enzyme, at pH 8.0 and 37°C Glycine max
2.38
-
 formate mutant enzyme A267M, at pH 8.0 and 37°C Glycine max
140
-
 NAD+ mutant enzyme A267M/I272V/F290D, at pH 8.0 and 37°C Glycine max
170
-
 NAD+ mutant enzyme A267M/I272V, at pH 8.0 and 37°C Glycine max
200
-
 NAD+ mutant enzyme F290D, at pH 8.0 and 37°C Glycine max
220
-
 NAD+ wild type enzyme, at pH 8.0 and 37°C Glycine max
230
-
 NAD+ mutant enzyme A267M/I272V/F290N, at pH 8.0 and 37°C Glycine max
230
-
 NAD+ mutant enzyme A267M/I272V/F290S, at pH 8.0 and 37°C Glycine max
400
-
 NAD+ mutant enzyme F290N, at pH 8.0 and 37°C Glycine max
450
-
 NAD+ mutant enzyme F290S, at pH 8.0 and 37°C Glycine max
510
-
 NAD+ mutant enzyme A267M, at pH 8.0 and 37°C Glycine max