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Literature summary for 1.17.1.9 extracted from

  • Ordu, E.; Yelboga, E.; Secundo, F.; Sessions, R.; Karagüler, N.
    The effect of methionine to cysteine substitution on the stability of formate dehydrogenase from Candida methylica (2012), J. Mol. Catal. B, 82, 109-114.
No PubMed abstract available
Search for more literature for 1.17.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM105 cells [Candida] methylica

Protein Variants

Protein Variants Comment Organism
D62C the mutant has 6% of the activity of the native enzyme in reducing conditions, but it has no observable activity in oxidizing conditions [Candida] methylica
M1C the catalytic efficiency (kcat/KM) of the mutant is 63% better than that of the native enzyme and the Tm value of this mutant is significantly higher than that of the native enzyme. The affinity of the mutant for formate is increased 31% than that of the native enzyme [Candida] methylica
M1C/D62C the mutant has only 3.75% and 1.6% of the catalytic efficiency of the native enzyme in oxidizing and reducing conditions, respectively [Candida] methylica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formate + NAD+ [Candida] methylica
-
 CO2 + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
[Candida] methylica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography [Candida] methylica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
 [Candida] methylica CO2 + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
FDH
-
 [Candida] methylica
NAD+-dependent formate dehydrogenase
-
 [Candida] methylica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 50 the enzyme remains stable from 25-50°C and rapidly loses activity at higher temperatures (inactive at 60°C) [Candida] methylica

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 [Candida] methylica