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Literature summary for 1.17.1.9 extracted from

  • Yu, S.; Zhu, L.; Zhou, C.; An, T.; Zhang, T.; Jiang, B.; Mu, W.
    Promising properties of a formate dehydrogenase from a methanol-assimilating yeast Ogataea parapolymorpha DL-1 in His-tagged form (2013), Appl. Microbiol. Biotechnol., 98, 1621-1630.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Ogataea angusta

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM Cu2+ inhibits the enzyme activity by 79% Ogataea angusta
Ni2+ 1 mM Ni2+ inhibits the enzyme activity by 28% Ogataea angusta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.083
-
NAD+ at pH 6.5 and 30°C Ogataea angusta
0.82
-
formate at pH 6.5 and 30°C Ogataea angusta

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM Ca2+ improves the enzyme activity by 77% Ogataea angusta
Co2+ 1 mM Co2+ improves the enzyme activity by 46% Ogataea angusta
additional information Mn2+, Ba2+, and Mg2+ do not significantly change the enzyme activity Ogataea angusta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39994
-
2 * 39994, calculated from amino acid sequence Ogataea angusta
40000
-
2 * 40000, SDS-PAGE Ogataea angusta
78000
-
native enzyme, gel filtration Ogataea angusta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formate + NAD+ Ogataea angusta
-
CO2 + NADH + H+
-
?
formate + NAD+ Ogataea angusta DL-1
-
CO2 + NADH + H+
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
dimethyl sulfoxide the enzyme activity almost does not decrease after incubation in 20% (v/v) dimethyl sulfoxide Ogataea angusta
n-Butanol the enzyme activity almost does not decrease after incubation in 20% (v/v) n-butanol Ogataea angusta
n-hexane the enzyme activity almost does not decrease after incubation in 20% (v/v) n-hexane Ogataea angusta

Organism

Organism UniProt Comment Textmining
Ogataea angusta P33677 formerly Ogataea parapolymorpha
-
Ogataea angusta DL-1 P33677 formerly Ogataea parapolymorpha
-

Purification (Commentary)

Purification (Comment) Organism
nickel chelating Sepharose column chromatography Ogataea angusta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
Ogataea angusta CO2 + NADH + H+
-
?
formate + NAD+
-
Ogataea angusta DL-1 CO2 + NADH + H+
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 40000, SDS-PAGE Ogataea angusta
homodimer 2 * 39994, calculated from amino acid sequence Ogataea angusta

Synonyms

Synonyms Comment Organism
FDH
-
Ogataea angusta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Ogataea angusta

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 70 the enzyme has more than 70% of relative activity when the temperature is between 60 and 70°C, but the enzyme activity decreases abruptly when the temperature is controlled below 60°C or above 70 °C Ogataea angusta

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 the enzyme retains 93, 88, 83, and 71% of its initial activity after 4 h of exposure at 40, 50, 55, and 60 °C, respectively Ogataea angusta

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.32
-
formate at pH 6.5 and 30°C Ogataea angusta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
Ogataea angusta

pH Range

pH Minimum pH Maximum Comment Organism
6 8 the enzyme displays a relatively wide pH spectrum and shows more than 98% of relative activity from pH 6.0 to 7.0 and still has 81 and 75% of relative activity under pH 7.5 and 8.0, respectively Ogataea angusta

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Ogataea angusta

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.83
-
formate at pH 6.5 and 30°C Ogataea angusta