Literature summary for 1.17.1.9 extracted from

Bekhouche, M.; Doumeche, B.; Blum, L.
Chemical modifications by ionic liquid-inspired cations improve the activity and the stability of formate dehydrogenase in [MMIm][Me2PO4] (2010), J. Mol. Catal. B, 65, 73-78.

No PubMed abstract available

General Stability

General Stability	Organism
after modification with either cholinium, hydroxyethyl-methylimidazolium, or hydroxypropyl-methylimidazolium cations, the FDH still presents 30-45% of activity in aqueous buffer in 70% (v/v) dimethylimidazolium dimethyl phosphate while the native enzyme is inactive at this ionic liquid concentration. The half-life of the modified enzyme is also increased by a 5fold factor after modification by cholinium (9 days) and by a 3fold factor after modification by hydroxyethyl-methylimidazolium or hydroxypropyl-methylimidazolium cations (6 and 5 days respectively) in aqueous solution. The half-life of the wild type FDH in aqueous solution is 1.5 days. When stored in 37.5% (v/v) dimethylimidazolium dimethyl phosphate, both modified and unmodified FDH have an increased half-life of 6-9 days	[Candida] boidinii

Organism

after modification with either cholinium, hydroxyethyl-methylimidazolium, or hydroxypropyl-methylimidazolium cations, the FDH still presents 30-45% of activity in aqueous buffer in 70% (v/v) dimethylimidazolium dimethyl phosphate while the native enzyme is inactive at this ionic liquid concentration. The half-life of the modified enzyme is also increased by a 5fold factor after modification by cholinium (9 days) and by a 3fold factor after modification by hydroxyethyl-methylimidazolium or hydroxypropyl-methylimidazolium cations (6 and 5 days respectively) in aqueous solution. The half-life of the wild type FDH in aqueous solution is 1.5 days. When stored in 37.5% (v/v) dimethylimidazolium dimethyl phosphate, both modified and unmodified FDH have an increased half-life of 6-9 days

[Candida] boidinii

Inhibitors

Inhibitors	Comment	Organism	Structure
dimethylimidazolium dimethyl phosphate	FDh is inactivated and unstable in the presence of high concentration (above 50%) of the water soluble dimethylimidazolium dimethyl phosphate ionic liquid	[Candida] boidinii

Organism

Organism	UniProt	Comment	Textmining
[Candida] boidinii	O13437	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
formate + NAD+	-	[Candida] boidinii	CO2 + NADH + H+	-	?

Synonyms

Synonyms	Comment	Organism
FDH	-	[Candida] boidinii

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	[Candida] boidinii