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Literature summary for 1.17.1.9 extracted from

  • Andreadeli, A.; Flemetakis, E.; Axarli, I.; Dimou, M.; Udvardi, M.K.; Katinakis, P.; Labrou, N.E.
    Cloning and characterization of Lotus japonicus formate dehydrogenase: a possible correlation with hypoxia (2009), Biochim. Biophys. Acta, 1794, 976-984.
    View publication on PubMed
Search for more literature for 1.17.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Lotus japonicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0259
-
 NAD+ in 0.1 M potassium phosphate buffer, pH 7.5, at 30°C Lotus japonicus
6.1
-
 formate in 0.1 M potassium phosphate buffer, pH 7.5, at 30°C Lotus japonicus
29.5
-
 NADP+ in 0.1 M potassium phosphate buffer, pH 7.5, at 30°C Lotus japonicus

Organism

Organism UniProt Comment Textmining
Lotus japonicus B6VPZ9
-
-

Purification (Commentary)

Purification (Comment) Organism
Cibacron blue 3GA-Sepharose column chromatography Lotus japonicus

Source Tissue

Source Tissue Comment Organism Textmining
root nodule detected in cells of the root vascular bundle adjacent to nodule, higher mRNA levels are detected in developing green seedpods and during the early stages of nodule development Lotus japonicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+ FDH1 exhibits absolute specificity for formate and NAD+ Lotus japonicus CO2 + NADH + H+
-
 ?
formate + NADP+ FDH1 uses NADP+ with low specificity compared to NAD+ Lotus japonicus CO2 + NADPH
-
 ?

Synonyms

Synonyms Comment Organism
FDH1
-
 Lotus japonicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
 NADP+ in 0.1 M potassium phosphate buffer, pH 7.5, at 30°C Lotus japonicus
1.2
-
 NAD+ in 0.1 M potassium phosphate buffer, pH 7.5, at 30°C Lotus japonicus
1.3
-
 formate in 0.1 M potassium phosphate buffer, pH 7.5, at 30°C Lotus japonicus

Cofactor

Cofactor Comment Organism Structure
NAD+ FDH1 shows activity using NAD+ or NADP+, FDH1 is highly specific to NAD+ compared to NADP+ Lotus japonicus
NADP+ FDH1 shows activity using NAD+ or NADP+, FDH1 is highly specific to NAD+ compared to NADP+ Lotus japonicus