Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Gelatoporia subvermispora |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | 86% inhibition at 50 mM; 90% inhibition at 50 mM | Gelatoporia subvermispora | |
ATP | 45% inhibition at 50 mM; 56% inhibition at 50 mM | Gelatoporia subvermispora | |
Cu2+ | 82 inhibition at 1 mM; complete inhibition at 1 mM | Gelatoporia subvermispora | |
Hg2+ | 98% inhibition at 1 mM; 98% inhibition at 1 mM | Gelatoporia subvermispora | |
NADH | 66% inhibition at 0.12 mM; 73% inhibition at 0.12 mM | Gelatoporia subvermispora | |
NADPH | 26% inhibition at 0.24 mM; 58% inhibition at 0.24 mM | Gelatoporia subvermispora | |
Sodium azide | 94% inhibition at 1 mM; 99% inhibition at 1 mM | Gelatoporia subvermispora |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.6 | - |
formate | isozyme FDH2 | Gelatoporia subvermispora | |
4.1 | - |
formate | isozyme FDH1 | Gelatoporia subvermispora | |
74.6 | - |
NAD+ | isozyme FDH1 | Gelatoporia subvermispora | |
140 | - |
NAD+ | isozyme FDH2 | Gelatoporia subvermispora |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no divalent metal cation is needed as a cofactor for enzyme activity | Gelatoporia subvermispora |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39300 | - |
predicted from amino acid sequence | Gelatoporia subvermispora |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gelatoporia subvermispora | A9ZNT8 | - |
- |
Gelatoporia subvermispora | A9ZNT9 | - |
- |
Gelatoporia subvermispora CS105 | A9ZNT8 | - |
- |
Gelatoporia subvermispora CS105 | A9ZNT9 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ column chromatography | Gelatoporia subvermispora |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | 100% specificity | Gelatoporia subvermispora | CO2 + NADH + H+ | - |
? | |
formate + NAD+ | 100% specificity | Gelatoporia subvermispora CS105 | CO2 + NADH + H+ | - |
? | |
additional information | no activity with malate, lactate, glycolate, pyruvate, and glyoxylate | Gelatoporia subvermispora | ? | - |
? | |
additional information | no activity with malate, lactate, glycolate, pyruvate, and glyoxylate | Gelatoporia subvermispora CS105 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FDB2 | isozyme | Gelatoporia subvermispora |
FDH1 | isozyme | Gelatoporia subvermispora |
NAD-dependent formate dehydrogenase | - |
Gelatoporia subvermispora |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Gelatoporia subvermispora |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Gelatoporia subvermispora | predicted from amino acid sequence | - |
6.29 |