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Literature summary for 1.17.1.9 extracted from

  • Nanba, H.; Takaoka, Y.; Hasegawa, J.
    Purification and characterization of formate dehydrogenase from Ancylobacter aquaticus strain KNK607M, and cloning of the gene (2003), Biosci. Biotechnol. Biochem., 67, 720-728.
    View publication on PubMed
Search for more literature for 1.17.1.9

Cloned(Commentary)

Cloned (Comment) Organism
high expression in Escherichia coli using the expression plasmid pFA002 Ancylobacter aquaticus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 37% inhibition Ancylobacter aquaticus
Hg2+ 1 mM, complete inhibition Ancylobacter aquaticus
NaN3 1 mM, complete inhibition Ancylobacter aquaticus
NEM 1 mM, 25.3% inhibition Ancylobacter aquaticus
PCMB 0.2 mM, complete inhibition Ancylobacter aquaticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.057
-
 NAD+ pH 7.0, 30°C Ancylobacter aquaticus
2.4
-
 formate pH 7.0, 30°C Ancylobacter aquaticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 2 * 44000, SDS-PAGE Ancylobacter aquaticus
100000
-
 gel filtration Ancylobacter aquaticus

Organism

Organism UniProt Comment Textmining
Ancylobacter aquaticus Q845T0
-
-
Ancylobacter aquaticus KNK607M Q845T0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ancylobacter aquaticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.47
-
-
 Ancylobacter aquaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
 Ancylobacter aquaticus CO2 + NADH + H+
-
 ?
formate + NAD+
-
 Ancylobacter aquaticus KNK607M CO2 + NADH + H+
-
 ?
formate + NADP+ activity with NADP+ is 2.4% of the activity with NAD+ Ancylobacter aquaticus CO2 + NADPH
-
 ?
formate + NADP+ activity with NADP+ is 2.4% of the activity with NAD+ Ancylobacter aquaticus KNK607M CO2 + NADPH
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 44000, SDS-PAGE Ancylobacter aquaticus

Synonyms

Synonyms Comment Organism
NAD-dependent formate dehydrogenase
-
 Ancylobacter aquaticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Ancylobacter aquaticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 60 35°C: about 50% of maximal activity, 60°C: about 45% of maximal activity Ancylobacter aquaticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 7.0, 10 min, stable at or lower Ancylobacter aquaticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
-
 Ancylobacter aquaticus

pH Range

pH Minimum pH Maximum Comment Organism
5 10.5 pH 5.0: about 60% of maximal activity, pH 5.3: about 80% of maximal activity, pH 9.6: about 80% of maximal activity, pH 10.5: about 65% of maximal activity Ancylobacter aquaticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 30°C, 20 h, about 50% of initial activity Ancylobacter aquaticus
7
-
 30°C, 20 h, most stable at Ancylobacter aquaticus
8
-
 30°C, 20 h, about 25% of initial activity Ancylobacter aquaticus
8.5
-
 30°C, 20 h, about 40% of initial activity Ancylobacter aquaticus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Ancylobacter aquaticus
NADP+ activity is 2.4% of the activity with NAD+ Ancylobacter aquaticus