Protein Variants | Comment | Organism |
---|---|---|
E61K | rate constant of thermal inactivation at 54°C, 60°C, 62°C and 65°C is decreased compared to wild-type enzyme | Mycolicibacterium vaccae |
E61P | rate constant of thermal inactivation at 54°C, 60°C, 62°C and 65°C is decreased compared to wild-type enzyme | Mycolicibacterium vaccae |
E61Q | rate constant of thermal inactivation at 54°C, 60°C, 62°C and 65°C is decreased compared to wild-type enzyme | Mycolicibacterium vaccae |
K61R | rate constant of thermal inactivation at 54°C and at 60°C is slightly decreased, at 62°C, and 65°C the constant is increased compared to wild-type enzyme | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium vaccae | - |
N10 | - |
Mycolicibacterium vaccae N10 | - |
N10 | - |
Pseudomonas sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | - |
Pseudomonas sp. | CO2 + NADH + H+ | - |
? | |
formate + NAD+ | - |
Mycolicibacterium vaccae | CO2 + NADH + H+ | - |
? | |
formate + NAD+ | - |
Mycolicibacterium vaccae N10 | CO2 + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MycFDH | - |
Mycolicibacterium vaccae |
PseFDH | - |
Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
54 | - |
rate constant of thermal inactivation is 0.00000504 per sec for wild-type enzyme and 0.00000463 per sec for mutant enzyme K61R | Pseudomonas sp. |
54 | - |
rate constant of thermal inactivation is 0.0000104 per sec for wild-type enzyme, 0.0000093 per sec for mutant enzyme E61Q, 0.00000754 per sec for mutant enzyme E61P and 0.00000766 per sec for mutant enzyme E61K | Mycolicibacterium vaccae |
60 | - |
rate constant of thermal inactivation is 0.0000755 per sec for wild-type enzyme and 0.000071 per sec for mutant enzyme K61R | Pseudomonas sp. |
60 | - |
rate constant of thermal inactivation is per 0.000336 sec for wild-type enzyme, 0.000174 per sec for mutant enzyme E61Q, 0.000115 per sec for mutant enzyme E61P and 0.000117 per sec for mutant enzyme E61K | Mycolicibacterium vaccae |
62 | - |
rate constant of thermal inactivation is 0.000155 per sec for wild-type enzyme and 0.000212 per sec for mutant enzyme K61R | Pseudomonas sp. |
62 | - |
rate constant of thermal inactivation is per 0.000924 sec for wild-type enzyme, 0.000590 per sec for mutant enzyme E61Q, 0.000335 per sec for mutant enzyme E61P and 0.0004 per sec for mutant enzyme E61K | Mycolicibacterium vaccae |
63 | - |
rate constant of thermal inactivation is 0.000320 per sec for wild-type enzyme and 0.00000463 per sec for mutant enzyme K61R | Pseudomonas sp. |
65 | - |
rate constant of thermal inactivation is 0.000774 per sec for wild-type enzyme and 0.000813 per sec for mutant enzyme K61R | Pseudomonas sp. |
65 | - |
rate constant of thermal inactivation is per 0.002860 sec for wild-type enzyme, 0.00224 per sec for mutant enzyme E61Q, 0.0012 per sec for mutant enzyme E61P and 0.00131 per sec for mutant enzyme E61K | Mycolicibacterium vaccae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas sp. | |
NAD+ | - |
Mycolicibacterium vaccae |