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Literature summary for 1.17.1.9 extracted from

  • Allais, J.J.; Louktibi, A.; Baratti, J.
    Oxidation of methanol by the yeast Pichia pastoris: purification and properties of the formate dehydrogenase (1983), Agric. Biol. Chem., 47, 2547-2554.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
CN-
-
Komagataella pastoris
Cu2+
-
Komagataella pastoris
Hg2+
-
Komagataella pastoris
N3-
-
Komagataella pastoris
NADH
-
Komagataella pastoris
NO2- 1 mM, 5% inhibition Komagataella pastoris
NO3- 1 mM, 13% inhibition Komagataella pastoris
PCMB
-
Komagataella pastoris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
NAD+
-
Komagataella pastoris
15
-
formate
-
Komagataella pastoris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
2 * 34000, SDS-PAGE Komagataella pastoris
70000
-
gel filtration Komagataella pastoris

Organism

Organism UniProt Comment Textmining
Komagataella pastoris
-
IFP 206
-
Komagataella pastoris IFP 206
-
IFP 206
-

Purification (Commentary)

Purification (Comment) Organism
-
Komagataella pastoris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Komagataella pastoris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
Komagataella pastoris CO2 + NADH + H+
-
?
formate + NAD+
-
Komagataella pastoris IFP 206 CO2 + NADH + H+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 34000, SDS-PAGE Komagataella pastoris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
47
-
-
Komagataella pastoris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
54
-
10 min, 50% loss of activity Komagataella pastoris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Komagataella pastoris

pH Range

pH Minimum pH Maximum Comment Organism
5.3 10.1 50% of maximal activity at pH 5.3 and at pH 10.1 Komagataella pastoris

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.2
-
37°C, 15 min, 50% loss of activity Komagataella pastoris
9.9
-
37°C, 15 min, 50% loss of activity Komagataella pastoris

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Komagataella pastoris