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Literature summary for 1.17.1.9 extracted from
- S-Formylgluthathione: the substrate for formate dehydrogenase in methanol-utilizing yeasts (1976), Arch. Microbiol., 111, 77-83.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
NAD+ | - |
Ogataea angusta |  |
| 1.1 | - |
S-formylglutathione | - |
Ogataea angusta | |
| 40 | - |
formate | - |
Ogataea angusta | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Ogataea angusta | S-formylglutathione rather than free formate is an intermediate in oxidation of methanol by yeast | ? | - |
? | |
| additional information | Ogataea pini | S-formylglutathione rather than free formate is an intermediate in oxidation of methanol by yeast | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ogataea angusta | - |
- |
- |
| Ogataea pini | - |
- |
- |
| [Candida] boidinii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ogataea angusta |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.83 | - |
- |
Ogataea angusta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formate + NAD+ | - |
[Candida] boidinii | CO2 + NADH + H+ | - |
? | |
| formate + NAD+ | - |
Ogataea angusta | CO2 + NADH + H+ | - |
? | |
| formate + NAD+ | - |
Ogataea pini | CO2 + NADH + H+ | - |
? | |
| additional information | S-formylglutathione rather than free formate is an intermediate in oxidation of methanol by yeast | Ogataea angusta | ? | - |
? | |
| additional information | S-formylglutathione rather than free formate is an intermediate in oxidation of methanol by yeast | Ogataea pini | ? | - |
? | |
| S-formylglutathione + NAD+ | about 40fold lower Km-value than with formate | Ogataea angusta | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Ogataea angusta |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Ogataea angusta |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
[Candida] boidinii | |
| NAD+ | - |
Ogataea angusta | |
| NAD+ | - |
Ogataea pini | |
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Release 2023.1 (January 2023)
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