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Literature summary for 1.17.1.9 extracted from

  • Galkin, A.G.; Kutsenko, A.S.; Bajulina, N.P.; Esipova, N.G.; Lamzin, V.S.; Mesentsev, A.V.; Shelukho, D.V.; Tikhonova, T.V.; Tishkov, V.I.; Ustinnikova, T.B.; Popov, V.O.
    Site-directed mutagenesis of the essential arginine of the formate dehydrogenase active centre (2001), Biochim. Biophys. Acta, 1594, 136-149.
    View publication on PubMed
Search for more literature for 1.17.1.9

Protein Variants

Protein Variants Comment Organism
R284A change of the catalytic, thermodynamic and spectral properties of the enzyme. The affinity of the mutants for the substrate formate or the transition state analogue azide decreases Pseudomonas sp.
R284A mutant enzyme is completely inactive Pseudomonas sp.
R284Q change of the catalytic, thermodynamic and spectral properties of the enzyme. The affinity of the mutants for the substrate formate or the transition state analogue azide decreases Pseudomonas sp.
R284Q Km-value for formate and Ki-value for azide decreases, KM-value for NAD+ is not affected Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
azide
-
 Pseudomonas sp.
N3-
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 NAD+ pH 7.0, 37°C, wild-type enzyme Pseudomonas sp.
0.17
-
 formate pH 7.0, 37°C, mutant enzyme R284Q Pseudomonas sp.
15
-
 formate pH 7.0, 37°C, wild-type enzyme Pseudomonas sp.
145
-
 formate pH 7.0, 37°C, mutant enzyme R284Q Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Synonyms

Synonyms Comment Organism
formate dehydrogenase
-
 Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.31
-
 formate pH 7.0, 37°C, mutant enzyme R284Q Pseudomonas sp.
10
-
 formate pH 7.0, 37°C, wild-type enzyme Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00015
-
 azide pH 7.0, 37°C, wild-type enzyme Pseudomonas sp.
0.024
-
 azide pH 7.0, 37°C, mutant enzyme R284Q Pseudomonas sp.