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Literature summary for 1.17.1.9 extracted from

  • Kearny, J.J.; Sagers, R.D.
    Formate dehydrogenase from Clostridium acidiurici (1972), J. Bacteriol., 109, 152-161.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.1.9

General Stability

General Stability Organism
instability to freezing and thawing Gottschalkia acidurici
strong inhibition by light Gottschalkia acidurici

Inhibitors

Inhibitors Comment Organism Structure
CN- 0.1 mM NaCN, 90% inhibition Gottschalkia acidurici
EDTA 50 mM, slight inhibition Gottschalkia acidurici

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 benzyl viologen
-
 Gottschalkia acidurici
3.1
-
 formate
-
 Gottschalkia acidurici

Organism

Organism UniProt Comment Textmining
Gottschalkia acidurici
-
-
-

Oxidation Stability

Oxidation Stability Organism
strong inhibition by O2 Gottschalkia acidurici

Purification (Commentary)

Purification (Comment) Organism
-
 Gottschalkia acidurici

Storage Stability

Storage Stability Organism
5°C, in the dark, in 0.05 M potassium phosphate buffer, pH 7, 1 mM EDTA, 5 mM 2-mercaptoethanol, 10% ammonium sulfate, stable for at least 3 weeks Gottschalkia acidurici

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + benzyl viologen
-
 Gottschalkia acidurici CO2 + reduced benzyl viologen
-
 ?
formate + NAD+
-
 Gottschalkia acidurici CO2 + NADH + H+
-
 ir

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8.2
-
 Gottschalkia acidurici

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Gottschalkia acidurici