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Literature summary for 1.17.1.9 extracted from

  • Quayle, J.R.
    Formate dehydrogenase (1966), Methods Enzymol., 9, 360-364.
No PubMed abstract available
Search for more literature for 1.17.1.9

Application

Application Comment Organism
analysis enzyme may be used for direct spectrophotometric assay of formate Cupriavidus oxalaticus

Inhibitors

Inhibitors Comment Organism Structure
CN- 1 mM KCN, complete inhibition Cupriavidus oxalaticus
KF 1 mM, 80% inhibition Cupriavidus oxalaticus
N3- 1 mM NaN3, 90% inhibition Cupriavidus oxalaticus
NaN3 1 mM, 80% inhibition Cupriavidus oxalaticus
sodium hypophosphite 1 mM, 80% inhibition Cupriavidus oxalaticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.077
-
 formate
-
 Cupriavidus oxalaticus
0.64
-
 NAD+
-
 Cupriavidus oxalaticus

Organism

Organism UniProt Comment Textmining
Cupriavidus oxalaticus
-
-
-
Cupriavidus oxalaticus OX1
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.46
-
-
 Cupriavidus oxalaticus

Storage Stability

Storage Stability Organism
-15°C, 50% loss of activity after 1 month Cupriavidus oxalaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+ equilibrium strongly favors dehydrogenation of formate Cupriavidus oxalaticus CO2 + NADH + H+
-
 r
formate + NAD+ equilibrium strongly favors dehydrogenation of formate Cupriavidus oxalaticus OX1 CO2 + NADH + H+
-
 r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
 Cupriavidus oxalaticus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Cupriavidus oxalaticus