Literature summary for 1.17.1.8 extracted from

Dommaraju, S.; Gorman, M.; Dogovski, C.; Pearce, F.; Gerrard, J.; Dobson, R.; Parker, M.; Perugini, M.
Cloning, expression and crystallization of dihydrodipicolinate reductase from methicillin-resistant Staphylococcus aureus (2009), Acta Crystallogr. Sect. F, 66, 57-60.

Cloned(Commentary)

Cloned (Comment)	Organism
gene dapB from strain MRSA252, expression in Escherichia coli BL21(DE3)	Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, sitting-drop vapour-diffusion method, 100 ml drops of 9-10 mg/ml protein in 20 mM Tris-HCl, pH 8.0, and 150 mM NaCl with 10 mM 2,6-PDC and 10 mM NADPH are mixed with 100 ml of reservoir solution containing 2.4 M ammonium sulfate, 0.2 M sodium fluoride, and 0.1 M bis-tris propane, pH 7.5, and ethanol added to 10% v/v, 8°C, X-ray diffraction structure determination and analysis at 3.65 A resolution	Staphylococcus aureus

Crystallization (Comment)

Organism

purified recombinant enzyme, sitting-drop vapour-diffusion method, 100 ml drops of 9-10 mg/ml protein in 20 mM Tris-HCl, pH 8.0, and 150 mM NaCl with 10 mM 2,6-PDC and 10 mM NADPH are mixed with 100 ml of reservoir solution containing 2.4 M ammonium sulfate, 0.2 M sodium fluoride, and 0.1 M bis-tris propane, pH 7.5, and ethanol added to 10% v/v, 8°C, X-ray diffraction structure determination and analysis at 3.65 A resolution

Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
26668	-	4 * 26668, mass spectrometry and gel filtration	Staphylococcus aureus

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	-	methicillin-resistant strain, MRSA-DHDPR, gene dapB	-
Staphylococcus aureus MRSA252	-	methicillin-resistant strain, MRSA-DHDPR, gene dapB	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme 5.9fold from Escherichia coli BL21(DE3) by ion-exchange and hydrophobic interaction chromatography, and gel filtration	Staphylococcus aureus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
10.1	-	purified recombinant enzyme, pH not specified in the publication, temperature not specified in the publication	Staphylococcus aureus

Subunits

Subunits	Comment	Organism
tetramer	4 * 26668, mass spectrometry and gel filtration	Staphylococcus aureus

Synonyms

Synonyms	Comment	Organism
DHDPR	-	Staphylococcus aureus
MRSA-DHDPR	-	Staphylococcus aureus

General Information

General Information	Comment	Organism
metabolism	DHDPR catalyzes the NADH/NADPH-dependent second step of the lysine-biosynthesis pathway in bacteria and plants	Staphylococcus aureus