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Literature summary for 1.17.1.8 extracted from
- Dihydrodipicolinate reductases from Bacillus cereus and Bacillus megaterium (1977), J. Biochem., 81, 1367-1373.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no flavins | Priestia megaterium | |
| additional information | no flavins | Bacillus cereus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dipicolinate | non-competitively versus dihydrodipicolinate, competitive versus NADPH | Bacillus cereus |  |
| dipicolinate | non-competitively versus dihydrodipicolinate, competitive versus NADPH | Priestia megaterium | |
| additional information | no inhibition by lysine and diaminopimelate | Bacillus cereus | |
| additional information | no inhibition by lysine and diaminopimelate | Priestia megaterium | |
| NADP+ | - |
Bacillus cereus | |
| NADP+ | - |
Priestia megaterium | |
| pyridine 2,6-dicarboxylic acid | - |
Bacillus cereus | |
| pyridine 2,6-dicarboxylic acid | - |
Priestia megaterium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
NADPH | - |
Bacillus cereus | |
| 0.013 | - |
NADPH | - |
Priestia megaterium | |
| 0.059 | - |
dihydrodipicolinate | - |
Priestia megaterium | |
| 0.062 | - |
dihydrodipicolinate | - |
Bacillus cereus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 150000 | - |
gel filtration | Priestia megaterium |
| 155000 | - |
gel filtration | Bacillus cereus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-dihydrodipicolinate + NAD(P)H | Priestia megaterium | - |
2,3,4,5-tetrahydrodipicolinate + NAD(P)+ | - |
? | |
| 2,3-dihydrodipicolinate + NAD(P)H | Bacillus cereus | - |
2,3,4,5-tetrahydrodipicolinate + NAD(P)+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
- |
- |
| Priestia megaterium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Priestia megaterium |
- |
Bacillus cereus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.67 | - |
- |
Bacillus cereus |
| 6.86 | - |
- |
Priestia megaterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-dihydrodipicolinate + NAD(P)H | - |
Priestia megaterium | 2,3,4,5-tetrahydrodipicolinate + NAD(P)+ | - |
? | |
| 2,3-dihydrodipicolinate + NAD(P)H | - |
Bacillus cereus | 2,3,4,5-tetrahydrodipicolinate + NAD(P)+ | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Priestia megaterium |
| 30 | - |
assay at | Bacillus cereus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Priestia megaterium |
| 7.4 | - |
- |
Bacillus cereus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no flavins | Priestia megaterium | |
| additional information | no flavins | Bacillus cereus | |
| NADH | slight activity | Priestia megaterium | |
| NADH | slight activity | Bacillus cereus | |
| NADPH | 3-4 times more effective than NADH | Priestia megaterium | |
| NADPH | 3-4 times more effective than NADH | Bacillus cereus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.085 | - |
dipicolinate | - |
Bacillus cereus | |
| 0.14 | - |
dipicolinate | - |
Priestia megaterium | |
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