Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2,6-dichloroindophenol | after addition of an artificial electron acceptor such as 2,6-dichloroindophenol, the nicotinate hydroxylase activity is significantly increased | Pusillimonas sp. (ex Stolz et al. 2005) | |
phenazine methosulfate | after addition of an artificial electron acceptor such as phenazine methosulfate, PMS, the nicotinate hydroxylase activity is significantly increased | Pusillimonas sp. (ex Stolz et al. 2005) |
Cloned (Comment) | Organism |
---|---|
genes nahAB1B2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant enzyme expression in the non-nicotinic acid-degrading Shinella sp. strain HZN7. The recombinant HZN7-pBBR-nahAB1B2 converts nicotinic acid into equimolar 6-hydroxynicotinic acid, while the recombinants HZN7-pBBR-nahAB1 (lacking component B2) and HZN7-pBBRnahAB2 (lacking component B1) cannot convert nicotinic acid. Cell-free extracts of HZN7-pBBR-nahAB1B2 exhibit nicotinic acid hydroxylase activity | Pusillimonas sp. (ex Stolz et al. 2005) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0659 | - |
nicotinate | recombinant enzyme, with phenazine methosulfate, pH 7.0, 30°C | Pusillimonas sp. (ex Stolz et al. 2005) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinate + H2O + NAD+ | Pusillimonas sp. (ex Stolz et al. 2005) | - |
6-hydroxynicotinate + NADH + H+ | - |
? | |
nicotinate + H2O + NAD+ | Pusillimonas sp. (ex Stolz et al. 2005) T2 / CCTCC M2014272 | - |
6-hydroxynicotinate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pusillimonas sp. (ex Stolz et al. 2005) | - |
- |
- |
Pusillimonas sp. (ex Stolz et al. 2005) T2 / CCTCC M2014272 | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | strain T2 is capable of utilizing nicotinic acid as sole carbon and nitrogen source | Pusillimonas sp. (ex Stolz et al. 2005) | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | after addition of an artificial electron acceptor, such as phenazine methosulfate, or 2,6-dichloroindophenol, the nicotinate hydroxylase activity is significantly increased | Pusillimonas sp. (ex Stolz et al. 2005) | ? | - |
? | |
additional information | after addition of an artificial electron acceptor, such as phenazine methosulfate, or 2,6-dichloroindophenol, the nicotinate hydroxylase activity is significantly increased | Pusillimonas sp. (ex Stolz et al. 2005) T2 / CCTCC M2014272 | ? | - |
? | |
nicotinate + H2O + 2,6-dichloroindophenol | - |
Pusillimonas sp. (ex Stolz et al. 2005) | 6-hydroxynicotinate + reduced 2,6-dichloroindophenol | - |
? | |
nicotinate + H2O + 2,6-dichloroindophenol | - |
Pusillimonas sp. (ex Stolz et al. 2005) T2 / CCTCC M2014272 | 6-hydroxynicotinate + reduced 2,6-dichloroindophenol | - |
? | |
nicotinate + H2O + NAD+ | - |
Pusillimonas sp. (ex Stolz et al. 2005) | 6-hydroxynicotinate + NADH + H+ | - |
? | |
nicotinate + H2O + NAD+ | - |
Pusillimonas sp. (ex Stolz et al. 2005) T2 / CCTCC M2014272 | 6-hydroxynicotinate + NADH + H+ | - |
? | |
nicotinate + H2O + phenazine methosulfate | - |
Pusillimonas sp. (ex Stolz et al. 2005) | 6-hydroxynicotinate + reduced phenazine methosulfate | - |
? | |
nicotinate + H2O + phenazine methosulfate | - |
Pusillimonas sp. (ex Stolz et al. 2005) T2 / CCTCC M2014272 | 6-hydroxynicotinate + reduced phenazine methosulfate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NA hydroxylase | - |
Pusillimonas sp. (ex Stolz et al. 2005) |
NahAB1B2 | - |
Pusillimonas sp. (ex Stolz et al. 2005) |
nicotinic acid hydroxylase | - |
Pusillimonas sp. (ex Stolz et al. 2005) |
three-component NA hydroxylase | - |
Pusillimonas sp. (ex Stolz et al. 2005) |
three-component nicotinic acid hydroxylase | - |
Pusillimonas sp. (ex Stolz et al. 2005) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pusillimonas sp. (ex Stolz et al. 2005) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Pusillimonas sp. (ex Stolz et al. 2005) |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoenzyme | Pusillimonas sp. (ex Stolz et al. 2005) | |
additional information | no activity with FMN and NADP+ | Pusillimonas sp. (ex Stolz et al. 2005) | |
NAD+ | - |
Pusillimonas sp. (ex Stolz et al. 2005) |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the degradation of nicotinic acid | Pusillimonas sp. (ex Stolz et al. 2005) |