General Stability | Organism |
---|---|
membrane-bound enzyme is stable, but solubilized enzyme is very labile, 20 mM nicotinate stabilizes | Pseudomonas fluorescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-dipyridyl | 1 mM, 10 min at 35°C, 21% loss of activity | Pseudomonas fluorescens | |
Acriflavin | 1 mM, 10 min at 35°C, 22% loss of activity | Pseudomonas fluorescens | |
AgNO3 | 1 mM, 10 min at 35°C, complete loss of activity | Pseudomonas fluorescens | |
CuCl2 | 1 mM, 10 min at 35°C, complete loss of activity | Pseudomonas fluorescens | |
HgCl2 | 1 mM, 10 min at 35°C, complete loss of activity | Pseudomonas fluorescens | |
KCN | strong inhibition, partially restored by addition of phenazine methosulfate | Pseudomonas fluorescens | |
N-ethylmaleimide | 1 mM, 10 min at 35°C, complete loss of activity | Pseudomonas fluorescens | |
NaN3 | 1 mM, 10 min at 35°C, 17% loss of activity | Pseudomonas fluorescens | |
p-chloromercuribenzoate | 1 mM, 10 min at 35°C, complete loss of activity | Pseudomonas fluorescens | |
phenylhydrazine | 1 mM, 10 min at 35°C, 15% loss of activity | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
nicotinate | - |
Pseudomonas fluorescens | |
0.7 | - |
3-pyridinesulfonate | - |
Pseudomonas fluorescens | |
2.1 | - |
2-Hydroxynicotinate | - |
Pseudomonas fluorescens | |
9.8 | - |
2-pyrazinecarboxylate | - |
Pseudomonas fluorescens | |
150 | - |
3-Cyanopyridine | - |
Pseudomonas fluorescens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Pseudomonas fluorescens | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
1 * 80000, SDS-PAGE, in presence of 50 mM 2-mercaptoethanol | Pseudomonas fluorescens |
100000 | - |
gel filtration | Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinate + H2O + electron acceptor | Pseudomonas fluorescens | first step of nicotinate catabolism | 6-hydroxynicotinate + reduced electron acceptor | - |
? | |
nicotinate + H2O + electron acceptor | Pseudomonas fluorescens | enzyme is linked to the cytochrome respiratory chain, phenazine methosulfate may accelerate the electron transfer from the electron respiratory chain to oxygen | 6-hydroxynicotinate + reduced electron acceptor | - |
? | |
nicotinate + H2O + electron acceptor | Pseudomonas fluorescens TN5 | first step of nicotinate catabolism | 6-hydroxynicotinate + reduced electron acceptor | - |
? | |
nicotinate + H2O + electron acceptor | Pseudomonas fluorescens TN5 | enzyme is linked to the cytochrome respiratory chain, phenazine methosulfate may accelerate the electron transfer from the electron respiratory chain to oxygen | 6-hydroxynicotinate + reduced electron acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Delftia acidovorans | - |
- |
- |
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas fluorescens TN5 | - |
- |
- |
Serratia marcescens | - |
- |
- |
Serratia marcescens IFO 12648 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
126fold purification | Pseudomonas fluorescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1 | - |
2-hydroxynicotinate | Pseudomonas fluorescens |
12 | - |
3-cyanopyridine | Pseudomonas fluorescens |
104 | - |
3-pyridinesulfonate | Pseudomonas fluorescens |
170 | - |
2-pyrazinecarboxylate | Pseudomonas fluorescens |
672 | - |
nicotinate | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxynicotinate + H2O + electron acceptor | hydroxylation at a low rate | Pseudomonas fluorescens | ? | - |
? | |
2-pyrazinecarboxylate + H2O + electron acceptor | - |
Pseudomonas fluorescens | ? | - |
? | |
2-pyrazinecarboxylate + H2O + electron acceptor | - |
Pseudomonas fluorescens TN5 | ? | - |
? | |
3-cyanopyridine + H2O + electron acceptor | hydroxylation at a low rate | Pseudomonas fluorescens | ? | - |
? | |
3-pyridinesulfonate + H2O + electron acceptor | - |
Pseudomonas fluorescens | ? | - |
? | |
additional information | not as substrates: nicotinamide, methyl nicotinate, beta-picoline, 3-pyridylcarbinol, quinolinic acid, 3-pyridylacetic acid, 3-hydroxypyridine, 3-chloropyridine, not as electron acceptors: methylene blue, FAD, FMN, NAD+, NADP+, horse heart cytochrome c, 2,3,5-triphenyl-2H-tetrazolium, neutral red, safranin O, menadione | Pseudomonas fluorescens | ? | - |
? | |
additional information | not as substrates: nicotinamide, methyl nicotinate, beta-picoline, 3-pyridylcarbinol, quinolinic acid, 3-pyridylacetic acid, 3-hydroxypyridine, 3-chloropyridine, not as electron acceptors: methylene blue, FAD, FMN, NAD+, NADP+, horse heart cytochrome c, 2,3,5-triphenyl-2H-tetrazolium, neutral red, safranin O, menadione | Pseudomonas fluorescens TN5 | ? | - |
? | |
nicotinate + H2O + electron acceptor | - |
Serratia marcescens | 6-hydroxynicotinate + reduced electron acceptor | - |
? | |
nicotinate + H2O + electron acceptor | - |
Delftia acidovorans | 6-hydroxynicotinate + reduced electron acceptor | - |
? | |
nicotinate + H2O + electron acceptor | purified enzyme probably loses its own native electron acceptor during the course of solubilization and purification, electron acceptors: phenazine methosulfate, potassium ferricyanide and with lower activity nitro blue tetrazolium, negative polarity at the 3-position on the pyridine ring of the substrate is important for reaction | Pseudomonas fluorescens | 6-hydroxynicotinate + reduced electron acceptor | - |
? | |
nicotinate + H2O + electron acceptor | first step of nicotinate catabolism | Pseudomonas fluorescens | 6-hydroxynicotinate + reduced electron acceptor | - |
? | |
nicotinate + H2O + electron acceptor | enzyme is linked to the cytochrome respiratory chain, phenazine methosulfate may accelerate the electron transfer from the electron respiratory chain to oxygen | Pseudomonas fluorescens | 6-hydroxynicotinate + reduced electron acceptor | - |
? | |
nicotinate + H2O + electron acceptor | - |
Serratia marcescens IFO 12648 | 6-hydroxynicotinate + reduced electron acceptor | - |
? | |
nicotinate + H2O + electron acceptor | purified enzyme probably loses its own native electron acceptor during the course of solubilization and purification, electron acceptors: phenazine methosulfate, potassium ferricyanide and with lower activity nitro blue tetrazolium, negative polarity at the 3-position on the pyridine ring of the substrate is important for reaction | Pseudomonas fluorescens TN5 | 6-hydroxynicotinate + reduced electron acceptor | - |
? | |
nicotinate + H2O + electron acceptor | first step of nicotinate catabolism | Pseudomonas fluorescens TN5 | 6-hydroxynicotinate + reduced electron acceptor | - |
? | |
nicotinate + H2O + electron acceptor | enzyme is linked to the cytochrome respiratory chain, phenazine methosulfate may accelerate the electron transfer from the electron respiratory chain to oxygen | Pseudomonas fluorescens TN5 | 6-hydroxynicotinate + reduced electron acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 80000, SDS-PAGE, in presence of 50 mM 2-mercaptoethanol | Pseudomonas fluorescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Pseudomonas fluorescens |
50 | - |
- |
Pseudomonas fluorescens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
10 min in 0.1 M potassium phosphate buffer, pH 7, stable below | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
- |
Pseudomonas fluorescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9 | broad pH-range with maximum at pH 8.3 | Pseudomonas fluorescens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8.8 | 10 min at 50°C in various buffers, stable | Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cytochromes or flavoprotein components | Pseudomonas fluorescens | |
phenazine methosulfate | phenazine methosulfate-requiring enzyme | Pseudomonas fluorescens |