Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
to 1.7 A resolution. The active site of PaoABC is highly exposed to the surface with no aromatic residues and an arginine (subunit PaoC-R440) making a direct interaction with subunit PaoC-E692, which acts as a base catalyst | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
R40K | mutation in subunit PaoC, strong decrease in activity | Escherichia coli |
R440H | mutation in subunit PaoC, strong decrease in activity, crystallization data | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
benzaldehyde | mutant R440H, pH 5.5, 22°C | Escherichia coli | |
0.129 | - |
benzaldehyde | wild-type, pH 5.5, 22°C | Escherichia coli | |
0.159 | - |
benzaldehyde | wild-type, pH 5.0, 22°C | Escherichia coli | |
0.204 | - |
benzaldehyde | mutant R440K, pH 5.5, 22°C | Escherichia coli | |
0.288 | - |
benzaldehyde | mutant R440H, pH 5.0, 22°C | Escherichia coli | |
0.307 | - |
benzaldehyde | wild-type, pH 4.5, 22°C | Escherichia coli | |
0.361 | - |
benzaldehyde | mutant R440K, pH 5.0, 22°C | Escherichia coli | |
0.396 | - |
benzaldehyde | mutant R440H, pH 4.5, 22°C | Escherichia coli | |
0.514 | - |
benzaldehyde | mutant R440H, pH 4.0, 22°C | Escherichia coli | |
0.52 | - |
benzaldehyde | mutant R440K, pH 4.5, 22°C | Escherichia coli | |
0.659 | - |
benzaldehyde | wild-type, pH 4.0, 22°C | Escherichia coli | |
1.517 | - |
benzaldehyde | mutant R440K, pH 4.0, 22°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Molybdenum | molybdopterin-cytosine-dinucleotide localized in subunit PaoC | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P77165 and P77324 and P77489 | P77165 i.e. alpha-subunit, P77324 i.e. beta-subnit, P77489 i.e. gamma-subunit | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzaldehyde + ferricyanide + H2O | - |
Escherichia coli | benzoate + 2 ferrocyanide + 2 H+ | - |
? | |
cinnamaldehyde + H2O + acceptor | - |
Escherichia coli | cinnamic acid + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | crystallization data | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
PaoABC | - |
Escherichia coli |
YagR | - |
Escherichia coli |
YagS | - |
Escherichia coli |
YagT | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
41 | - |
benzaldehyde | mutant R440K, pH 5.5, 22°C | Escherichia coli | |
48.7 | - |
benzaldehyde | mutant R440H, pH 5.5, 22°C | Escherichia coli | |
80.5 | - |
benzaldehyde | mutant R440K, pH 5.0, 22°C | Escherichia coli | |
150 | - |
benzaldehyde | mutant R440H, pH 5.0, 22°C | Escherichia coli | |
150 | - |
benzaldehyde | wild-type, pH 5.5, 22°C | Escherichia coli | |
217 | - |
benzaldehyde | wild-type, pH 5.0, 22°C | Escherichia coli | |
293 | - |
benzaldehyde | mutant R440H, pH 4.5, 22°C | Escherichia coli | |
307 | - |
benzaldehyde | mutant R440K, pH 4.0, 22°C | Escherichia coli | |
325 | - |
benzaldehyde | mutant R440K, pH 4.5, 22°C | Escherichia coli | |
404 | - |
benzaldehyde | mutant R440H, pH 4.0, 22°C | Escherichia coli | |
486 | - |
benzaldehyde | wild-type, pH 4.5, 22°C | Escherichia coli | |
1097 | - |
benzaldehyde | wild-type, pH 4.0, 22°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | subunit PaoB exhibits a typical FAD binding motif | Escherichia coli | |
molybdopterin cytosine dinucleotide | localized in subunit PaoC | Escherichia coli | |
[2Fe-2S]-center | iron-sulfur subunit PaoA can be divided into two subdomains, each carrying one [2Fe-2S] cluster | Escherichia coli | |
[4Fe-4S]-center | cluster is anchored to an additional 40 residues subdomain of subunit PaoB | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
200 | - |
benzaldehyde | mutant R440K, pH 4.0, 22°C | Escherichia coli | |
200 | - |
benzaldehyde | mutant R440K, pH 5.5, 22°C | Escherichia coli | |
220 | - |
benzaldehyde | mutant R440K, pH 5.0, 22°C | Escherichia coli | |
520 | - |
benzaldehyde | mutant R440H, pH 5.0, 22°C | Escherichia coli | |
620 | - |
benzaldehyde | mutant R440K, pH 4.5, 22°C | Escherichia coli | |
740 | - |
benzaldehyde | mutant R440H, pH 4.5, 22°C | Escherichia coli | |
790 | - |
benzaldehyde | mutant R440H, pH 4.0, 22°C | Escherichia coli | |
810 | - |
benzaldehyde | mutant R440H, pH 5.5, 22°C | Escherichia coli | |
1170 | - |
benzaldehyde | wild-type, pH 5.5, 22°C | Escherichia coli | |
1370 | - |
benzaldehyde | wild-type, pH 5.0, 22°C | Escherichia coli | |
1580 | - |
benzaldehyde | wild-type, pH 4.5, 22°C | Escherichia coli | |
1660 | - |
benzaldehyde | wild-type, pH 4.0, 22°C | Escherichia coli |