Cloned (Comment) | Organism |
---|---|
XOR is cloned and heterologously expressed in Spodoptera frugiperda insect cells | Sus scrofa |
Protein Variants | Comment | Organism |
---|---|---|
D430H | Km (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) slightly increased compared to wild-type, Vmax (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) decreased compared to wild-type, Km (xanthine) slightly decreased compared to wild-type, Vmax (xanthine) slightly decreased to wild-type | Sus scrofa |
D431A | Km (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) slightly increased compared to wild-type, Vmax (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) increased compared to wild-type, Km (xanthine) slightly increased compared to wild-type, Vmax (xanthine) increased to wild-type | Sus scrofa |
G47A | Km and Vmax (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) increased compared to wild-type, Km and Vmax (xanthine) slightly increased compared to wild-type | Sus scrofa |
K1230A | Km (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) 2.5fold increased compared to wild-type, Vmax (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) decreased compared to wild-type, Km (xanthine) 2fold increased compared to wild-type, Vmax (xanthine) 2fold decreased to wild-type | Sus scrofa |
N352A | Km (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) slightly increased compared to wild-type, Vmax (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) slightly decreased compared to wild-type, Km (xanthine) slightly increased compared to wild-type, Vmax (xanthine) slightly decreased compared to wild-type | Sus scrofa |
R427E | Km (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) decreased compared to wild-type, Vmax (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) decreased compared to wild-type, Km (xanthine) slightly increased compared to wild-type, Vmax (xanthine) comparable to wild-type | Sus scrofa |
S1227A | Km (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) 2.5fold increased compared to wild-type, Vmax (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) decreased compared to wild-type, Km (xanthine) 2fold increased compared to wild-type, Vmax (xanthine) 2fold decreased to wild-type | Sus scrofa |
S360P | Km (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) increased compared to wild-type, Vmax (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) decreased compared to wild-type, Km (xanthine) 3fold increased compared to wild-type, Vmax (xanthine) 3fold decreased compared to wild-type | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
? | KM (mM) (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) (pH 7.4, 37°C), wild-type: 0.0582, mutant G47A: 0.06568, mutant N352A: 0.0601, mutant S360P: 0.09353, mutant R427E: 0.02086, mutant D430H: 0.06468, mutant D431A: 0.06129, mutant S1227A: 0.148, mutant K1230A: 0.162 | Sus scrofa | |
0.0213 | - |
xanthine | pH 7.4, 37°C, mutant D430H, Vmax (micromol/min/mg): 7.47 | Sus scrofa | |
0.02169 | - |
xanthine | pH 7.4, 37°C, wild-type, Vmax (micromol/min/mg): 7.74 | Sus scrofa | |
0.02172 | - |
xanthine | pH 7.4, 37°C, mutant D431A, Vmax (micromol/min/mg): 8.52 | Sus scrofa | |
0.02227 | - |
xanthine | pH 7.4, 37°C, mutant N352A, Vmax (micromol/min/mg): 7.69 | Sus scrofa | |
0.02324 | - |
xanthine | pH 7.4, 37°C, mutant R427E, Vmax (micromol/min/mg): 7.75 | Sus scrofa | |
0.02413 | - |
xanthine | pH 7.4, 37°C, mutant G47A, Vmax (micromol/min/mg): 7.91 | Sus scrofa | |
0.039 | - |
xanthine | pH 7.4, 37°C, mutant K1230A, Vmax (micromol/min/mg): 4.02 | Sus scrofa | |
0.0508 | - |
xanthine | pH 7.4, 37°C, mutant S1227A, Vmax (micromol/min/mg): 3.94 | Sus scrofa | |
0.06428 | - |
xanthine | pH 7.4, 37°C, mutant S360P, Vmax (micromol/min/mg): 2.83 | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
- |
Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | in the presence of xanthine and NAD+ the enzyme catalyses the oxidation of cyadox (2-cyano-N'-((E)-1,4-dioxido-2-quinazolinyl)methylene)acetohydrazide to the respective N-oxide | Sus scrofa | ? | - |
? | |
xanthine + NAD+ + H2O | - |
Sus scrofa | urate + NADH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
xanthine oxidoreductase | - |
Sus scrofa |
XOR | - |
Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Sus scrofa |