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Literature summary for 1.17.1.4 extracted from

  • Chen, C.; Cheng, G.; Hao, H.; Dai, M.; Wang, X.; Huang, L.; Liu, Z.; Yuan, Z.
    Mechanism of porcine liver xanthine oxidoreductase mediated N-oxide reduction of cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide as revealed by docking and mutagenesis studies (2013), PLoS ONE, 8, e73912.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
XOR is cloned and heterologously expressed in Spodoptera frugiperda insect cells Sus scrofa

Protein Variants

Protein Variants Comment Organism
D430H Km (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) slightly increased compared to wild-type, Vmax (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) decreased compared to wild-type, Km (xanthine) slightly decreased compared to wild-type, Vmax (xanthine) slightly decreased to wild-type Sus scrofa
D431A Km (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) slightly increased compared to wild-type, Vmax (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) increased compared to wild-type, Km (xanthine) slightly increased compared to wild-type, Vmax (xanthine) increased to wild-type Sus scrofa
G47A Km and Vmax (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) increased compared to wild-type, Km and Vmax (xanthine) slightly increased compared to wild-type Sus scrofa
K1230A Km (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) 2.5fold increased compared to wild-type, Vmax (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) decreased compared to wild-type, Km (xanthine) 2fold increased compared to wild-type, Vmax (xanthine) 2fold decreased to wild-type Sus scrofa
N352A Km (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) slightly increased compared to wild-type, Vmax (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) slightly decreased compared to wild-type, Km (xanthine) slightly increased compared to wild-type, Vmax (xanthine) slightly decreased compared to wild-type Sus scrofa
R427E Km (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) decreased compared to wild-type, Vmax (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) decreased compared to wild-type, Km (xanthine) slightly increased compared to wild-type, Vmax (xanthine) comparable to wild-type Sus scrofa
S1227A Km (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) 2.5fold increased compared to wild-type, Vmax (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) decreased compared to wild-type, Km (xanthine) 2fold increased compared to wild-type, Vmax (xanthine) 2fold decreased to wild-type Sus scrofa
S360P Km (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) increased compared to wild-type, Vmax (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) decreased compared to wild-type, Km (xanthine) 3fold increased compared to wild-type, Vmax (xanthine) 3fold decreased compared to wild-type Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
? KM (mM) (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) (pH 7.4, 37°C), wild-type: 0.0582, mutant G47A: 0.06568, mutant N352A: 0.0601, mutant S360P: 0.09353, mutant R427E: 0.02086, mutant D430H: 0.06468, mutant D431A: 0.06129, mutant S1227A: 0.148, mutant K1230A: 0.162 Sus scrofa
0.0213
-
xanthine pH 7.4, 37°C, mutant D430H, Vmax (micromol/min/mg): 7.47 Sus scrofa
0.02169
-
xanthine pH 7.4, 37°C, wild-type, Vmax (micromol/min/mg): 7.74 Sus scrofa
0.02172
-
xanthine pH 7.4, 37°C, mutant D431A, Vmax (micromol/min/mg): 8.52 Sus scrofa
0.02227
-
xanthine pH 7.4, 37°C, mutant N352A, Vmax (micromol/min/mg): 7.69 Sus scrofa
0.02324
-
xanthine pH 7.4, 37°C, mutant R427E, Vmax (micromol/min/mg): 7.75 Sus scrofa
0.02413
-
xanthine pH 7.4, 37°C, mutant G47A, Vmax (micromol/min/mg): 7.91 Sus scrofa
0.039
-
xanthine pH 7.4, 37°C, mutant K1230A, Vmax (micromol/min/mg): 4.02 Sus scrofa
0.0508
-
xanthine pH 7.4, 37°C, mutant S1227A, Vmax (micromol/min/mg): 3.94 Sus scrofa
0.06428
-
xanthine pH 7.4, 37°C, mutant S360P, Vmax (micromol/min/mg): 2.83 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
-
Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information in the presence of xanthine and NAD+ the enzyme catalyses the oxidation of cyadox (2-cyano-N'-((E)-1,4-dioxido-2-quinazolinyl)methylene)acetohydrazide to the respective N-oxide Sus scrofa ?
-
?
xanthine + NAD+ + H2O
-
Sus scrofa urate + NADH
-
?

Synonyms

Synonyms Comment Organism
xanthine oxidoreductase
-
Sus scrofa
XOR
-
Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Sus scrofa