Protein Variants | Comment | Organism |
---|---|---|
E232A | decrease in kcat value, increase in KM-value | Rhodobacter capsulatus |
E730A | no enzymic activity | Rhodobacter capsulatus |
E730D | no enzymic activity | Rhodobacter capsulatus |
E730Q | no enzymic activity | Rhodobacter capsulatus |
E730R | no enzymic activity | Rhodobacter capsulatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no substrate inhibition | Rhodobacter capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.064 | - |
xanthine | wild-type, pH 7.8, 25°C | Rhodobacter capsulatus | |
0.103 | - |
NAD+ | wild-type, pH 7.8, 25°C | Rhodobacter capsulatus | |
0.163 | - |
xanthine | mutant E232A, pH 7.8, 25°C | Rhodobacter capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xanthine + NAD+ + H2O | - |
Rhodobacter capsulatus | urate + NADH | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.4 | - |
xanthine | mutant E232A, pH 7.8, 25°C | Rhodobacter capsulatus | |
108 | - |
xanthine | wild-type, pH 7.8, 25°C | Rhodobacter capsulatus |