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Literature summary for 1.17.1.4 extracted from

  • Ivanov, N.V.; Hubalek, F.; Trani, M.; Edmondson, D.E.
    Factors involved in the assembly of a functional molybdopyranopterin center in recombinant Comamonas acidovorans xanthine dehydrogenase (2003), Eur. J. Biochem., 270, 4744-4754.
    View publication on PubMed

Application

Application Comment Organism
synthesis expression of genes xdhAB encoding the two subunits of enzyme, in Escherichia coli, produces active enzyme with moleybdenum content of 0.11-0.16 mol per alphabeta protomer and iron and FAD levels at stoichiometries similar to native enzyme. Coexpression of xdhAB genes with Pseudomonas aeruginosa xdhC gene increases level of molybdenum incorporated to a 1:1 stoichiometry and results in high levels of functional protein, up to 2284 units per mg and 8039 mg per l Delftia acidovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.066
-
xanthine coexpression of genes xdhAB, conditions of high aeration, pH 7.8, 25°C Delftia acidovorans
0.067
-
xanthine coexpression of genes xdhAB, conditions of low aeration, pH 7.8, 25°C Delftia acidovorans
0.085
-
xanthine coexpression of genes xdhABC, conditions of high aeration, pH 7.8, 25°C Delftia acidovorans
0.1
-
NAD+ coexpression of genes xdhABC, conditions of high aeration, pH 7.8, 25°C Delftia acidovorans
0.113
-
NAD+ coexpression of genes xdhABC, conditions of low aeration, pH 7.8, 25°C Delftia acidovorans
0.148
-
NAD+ coexpression of genes xdhAB, conditions of high aeration, pH 7.8, 25°C Delftia acidovorans
0.156
-
NAD+ coexpression of genes xdhAB, conditions of low aeration, pH 7.8, 25°C Delftia acidovorans
80.09
-
xanthine coexpression of genes xdhABC, conditions of low aeration, pH 7.8, 25°C Delftia acidovorans

Organism

Organism UniProt Comment Textmining
Delftia acidovorans Q8RLC0 large subunit; expression of genes xdhAB encoding the two subunits of enzyme, in Escherichia coli, produces active enzyme. Coexpression of xdhAB genes with Pseudomonas aeruginosa xdhC gene results in high levels of functional protein
-
Delftia acidovorans Q8RLC1 small subunit; expression of genes xdhAB encoding the two subunits of enzyme, in Escherichia coli, produces active enzyme. Coexpression of xdhAB genes with Pseudomonas aeruginosa xdhC gene results in high levels of functional protein
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xanthine + NAD+ + H2O
-
Delftia acidovorans urate + NADH
-
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Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
xanthine coexpression of genes xdhAB, conditions of low aeration, pH 7.8, 25°C Delftia acidovorans
27
-
xanthine coexpression of genes xdhAB, conditions of high aeration, pH 7.8, 25°C Delftia acidovorans
86
-
xanthine coexpression of genes xdhABC, conditions of low aeration, pH 7.8, 25°C Delftia acidovorans
118
-
xanthine coexpression of genes xdhABC, conditions of high aeration, pH 7.8, 25°C Delftia acidovorans