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Literature summary for 1.17.1.4 extracted from

  • Parzen, S.D.; Fox, A.S.
    Purification of xanthine dehydrogenase from Drosophila melanogaster (1964), Biochim. Biophys. Acta, 92, 465-471.
    View publication on PubMed

General Stability

General Stability Organism
extremely labile Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
hypoxanthine cosubstrate: NAD+ Drosophila melanogaster
0.02
-
thio-NAD+ cosubstrate: hypoxanthine Drosophila melanogaster
0.0236
-
xanthine cosubstrate: NAD+ Drosophila melanogaster
0.025
-
NAD+ cosubstrate: hypoxanthine Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hypoxanthine + NAD+ + H2O Drosophila melanogaster
-
xanthine + NADH + H+
-
?
xanthine + NAD+ + H2O Drosophila melanogaster involved in pteridine metabolism, 40% of activity compared to hypoxanthine urate + NADH
-
?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-

Purification (Commentary)

Purification (Comment) Organism
529fold purification, several (NH4)2SO4 precipitation steps Drosophila melanogaster

Source Tissue

Source Tissue Comment Organism Textmining
whole body
-
Drosophila melanogaster
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1328
-
-
Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hypoxanthine + NAD+ + H2O
-
Drosophila melanogaster xanthine + NADH + H+
-
?
hypoxanthine + thio-NAD+ + H2O
-
Drosophila melanogaster xanthine + thio-NADH
-
?
xanthine + NAD+ + H2O involved in pteridine metabolism, 40% of activity compared to hypoxanthine Drosophila melanogaster urate + NADH
-
?
xanthine + thio-NAD+ + H2O
-
Drosophila melanogaster urate + thio-NADH
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Drosophila melanogaster

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Drosophila melanogaster