General Stability | Organism |
---|---|
oxygen-linked activity rather unstable during storage at 4°C, ferricyanide-linked activity stable to freezing and thawing | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arsenite | 90% loss of the ferricyanide-linked activity in the presence of 1.78 mM | Pseudomonas putida | |
KCN | complete inactivation of oxygen-linked activity in 15 min, decline of NAD+-linked activity in 75 min, ferricyanide-linked activity completely stable | Pseudomonas putida | |
methanol | 50% inhibition in 3 min at 1.5 M | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mo | - |
Pseudomonas putida | |
molybdenum-center | - |
Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
x * 72000, SDS-PAGE | Pseudomonas putida |
255000 | - |
sucrose density gradient centrifugation, gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xanthine + NAD+ + H2O | Pseudomonas putida | NAD+-linked activity, very low activity towards molecular oxygen | urate + NADH | - |
? | |
xanthine + O2 + H2O | Pseudomonas putida | very low activity | urate + O2- + 2 H+ | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Methanol | sensitive to, half-life: 3 min at 1.5 M | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, mainly ferricyanide- and NAD+-linked activities, chromatography steps | Pseudomonas putida |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | 0.0025 mg/ml sodium tungstate in the growth medium reduces level of ferricyanide-linked enzyme to 3% | Pseudomonas putida | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
163 | - |
ferricyanide-linked activity | Pseudomonas putida |
Storage Stability | Organism |
---|---|
4°C, 1 week | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xanthine + 2,6-dichlorophenolindophenol + H2O | 11% of the activity compared to ferricyanide as electron acceptor | Pseudomonas putida | urate + ? | - |
? | |
xanthine + ferricyanide + H2O | specific ferricyanide-dependent activity, no activity with NAD+, NADP+, oxygen, cytochrome c, FAD or FMN | Pseudomonas putida | urate + ferrocyanide | - |
? | |
xanthine + NAD+ + H2O | NAD+-linked activity, very low activity towards molecular oxygen | Pseudomonas putida | urate + NADH | - |
? | |
xanthine + O2 + H2O | very low activity | Pseudomonas putida | urate + O2- + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 72000, SDS-PAGE | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ferricyanide | specific xanthine oxidizing activity | Pseudomonas putida | |
heme | ferricyanide-linked activity, no FAD | Pseudomonas putida | |
NAD+ | activity different from ferricyanide-linked activity | Pseudomonas putida |