Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | recovery from oxidase to dehydrogenase type | Bos taurus | |
DTT | recovery from oxidase to dehydrogenase type | Rattus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5-dithiobis-(2-nitrobenzoate) | conversion of dehydrogenase type D to oxidase type O due to modification of a limited number of critical sulfhydryl groups | Rattus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mo | - |
Rattus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
x * 150000, SDS-urea -PAGE | Rattus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xanthine + NAD+ + H2O | Bos taurus | NAD+-dependent dehydrogenase type D | urate + NADH | - |
? | |
xanthine + NAD+ + H2O | Rattus sp. | NAD+-dependent dehydrogenase type D | urate + NADH | - |
? | |
xanthine + NAD+ + O2 + H2O + H+ | Rattus sp. | heat-treated intermediate dehydrogenase/oxidase type O | urate + NADH + H2O2 | - |
? | |
xanthine + O2 + H2O | Bos taurus | - |
urate + O2- + 2 H+ | - |
? | |
xanthine + O2 + H2O | Rattus sp. | NAD+-independent trypsin-treated oxidase type O | urate + O2- + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Rattus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified as antibody complex | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus sp. | - |
milk | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + electron acceptor + H2O | conversion to the oxidase type O by trypsinization leads to 80-100% decrease in the oxidation rate of NADH, conversion to the oxidase type O by heat-treatment leads to a diminution of NADH oxidation | Rattus sp. | NAD+ + reduced electron acceptor | - |
? | |
xanthine + 2,6-dichlorophenolindophenol + H2O | - |
Bos taurus | urate + ? | - |
? | |
xanthine + 2,6-dichlorophenolindophenol + H2O | greatly enhanced activity for dehydrogenase type D and trypsin- or heat-treated oxidase types O | Rattus sp. | urate + ? | - |
? | |
xanthine + cytochrome c + H2O | enhanced activity for heat- and trypsin-treated oxidase types O | Rattus sp. | urate + reduced cytochrome c | - |
? | |
xanthine + ferricyanide + H2O | low activity for both dehydrogenase type D and oxidase type O | Rattus sp. | urate + ferrocyanide | - |
? | |
xanthine + methylene blue + H2O | enhanced oxidation of xanthine for dehydrogenase type D and trypsin- or heat-treated oxidase type O | Rattus sp. | urate + reduced methylene blue | - |
? | |
xanthine + NAD+ + H2O | NAD+-dependent dehydrogenase type D | Bos taurus | urate + NADH | - |
? | |
xanthine + NAD+ + H2O | NAD+-dependent dehydrogenase type D | Rattus sp. | urate + NADH | - |
? | |
xanthine + NAD+ + O2 + H2O + H+ | heat-treated intermediate dehydrogenase/oxidase type O | Rattus sp. | urate + NADH + H2O2 | - |
? | |
xanthine + O2 + H2O | - |
Bos taurus | urate + O2- + 2 H+ | - |
? | |
xanthine + O2 + H2O | NAD+-independent trypsin-treated oxidase type O | Rattus sp. | urate + O2- + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 150000, SDS-urea -PAGE | Rattus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
conversion to an intermediate dehydrogenase-oxidase type O form in 30 min | Rattus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Bos taurus | |
FAD | trypsin-treated oxidase type O shows absence of FAD, heat-treated oxidase type O shows small measurable FAD | Rattus sp. | |
NAD+ | - |
Bos taurus | |
NAD+ | - |
Rattus sp. |