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Literature summary for 1.17.1.4 extracted from

  • Montalbini, P.
    Purification and some properties of xanthine dehydrogenase from wheat leaves (1998), Plant Sci., 134, 89-102.
No PubMed abstract available
Search for more literature for 1.17.1.4

Activating Compound

Activating Compound Comment Organism Structure
Urate 30% activation of hypoxanthine-NAD+ activity Triticum aestivum

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxymercuribenzoate 76% inhibition of xanthine-NAD+-activity at 0.002 mM Triticum aestivum
diethyldithiocarbamate 72% inhibition of xanthine-NAD+-activity at 10 mM Triticum aestivum
NaCN 69% inhibition of xanthine-NAD+-activity at 3.3 mM Triticum aestivum
o-phenanthroline 12% inhibition of xanthine-NAD+-activity at 7.5 mM Triticum aestivum
Salicylhydroxamic acid 71% inhibition of xanthine-NAD+-activity at 5 mM Triticum aestivum
Urate 15% inhibition of xanthine-NAD+-activity Triticum aestivum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0041
-
 hypoxanthine electron acceptor: NAD+ Triticum aestivum
0.0056
-
 phenazine methosulfate electron donor: xanthine Triticum aestivum
0.0057
-
 NADH electron acceptor: nitroblue tetrazolium Triticum aestivum
0.0075
-
 hypoxanthine electron acceptor: nitroblue tetrazolium Triticum aestivum
0.0082
-
 xanthine electron acceptor: NAD+ Triticum aestivum
0.0086
-
 xanthine electron acceptor: nitroblue tetrazolium Triticum aestivum
0.0113
-
 nitroblue tetrazolium electron donor: xanthine Triticum aestivum
0.171
-
 NAD+ electron donor: xanthine Triticum aestivum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
350000
-
 native PAGE Triticum aestivum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-amino-4-hydroxy-pterin + NAD+ + H2O Triticum aestivum i.e. pterin isoxanthopterin + NADH
-
 ?
2-amino-4-hydroxy-pterin + NAD+ + H2O Triticum aestivum 11% of the activity compared to xanthine isoxanthopterin + NADH
-
 ?
purine + NAD+ + H2O Triticum aestivum poor substrate ? + NADH
-
 ?
xanthine + NAD+ + H2O Triticum aestivum strict dehydrogenase activity, no utilization of O2 urate + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Triticum aestivum
-
 wheat
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Triticum aestivum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.23
-
 xanthine-NAD+ assay Triticum aestivum

Storage Stability

Storage Stability Organism
4°C, 25 mM Tris-boric acid, pH 8.5, 2.5 mM EDTA, 2 mM 2-mercaptoethanol, 0.2 mM sodium molybdate, one year Triticum aestivum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-4-hydroxy-pterin + NAD+ + H2O i.e. pterin Triticum aestivum isoxanthopterin + NADH
-
 ?
2-amino-4-hydroxy-pterin + NAD+ + H2O 11% of the activity compared to xanthine Triticum aestivum isoxanthopterin + NADH
-
 ?
2-amino-4-hydroxypterin + NAD+ + H2O i.e. pterin Triticum aestivum isoxanthopterin + NADH
-
 ?
2-amino-4-hydroxypterin + NAD+ + H2O 11% of the activity compared to xanthine Triticum aestivum isoxanthopterin + NADH
-
 ?
2-amino-4-hydroxypterin + nitroblue tetrazolium + H2O very low activity Triticum aestivum isoxanthopterin + reduced nitroblue tetrazolium
-
 ?
2-amino-4-hydroxypterin + nitroblue tetrazolium + H2O i.e. pterin Triticum aestivum isoxanthopterin + reduced nitroblue tetrazolium
-
 ?
4-hydroxypyrazolo(3,4-d)pyrimidine + nitroblue tetrazolium + H2O very low activity Triticum aestivum 4,6-dihydroxypyrazolo(3,4-d)pyrimidine + reduced nitroblue tetrazolium
-
 ?
4-hydroxypyrazolo(3,4-d)pyrimidine + nitroblue tetrazolium + H2O i.e. allopurinol Triticum aestivum 4,6-dihydroxypyrazolo(3,4-d)pyrimidine + reduced nitroblue tetrazolium
-
 ?
acetaldehyde + NAD+ + H2O very low activity Triticum aestivum acetate + NADH + H+
-
 ?
acetaldehyde + nitroblue tetrazolium + H2O very low activity Triticum aestivum acetic acid + reduced nitroblue tetrazolium
-
 ?
benzaldehyde + nitroblue tetrazolium + H2O very low activity Triticum aestivum benzoate + reduced nitroblue tetrazolium
-
 ?
hypoxanthine + nitroblue tetrazolium
-
 Triticum aestivum xanthine + ?
-
 ?
NADH + electron acceptor + H2O electron acceptor: nitroblue tetrazolium Triticum aestivum NAD+ + reduced electron acceptor
-
 ?
NADPH + nitroblue tetrazolium + H2O diaphorase activity Triticum aestivum NADP+ + reduced nitroblue tetrazolium
-
 ?
purine + NAD+ + H2O poor substrate Triticum aestivum ? + NADH
-
 ?
purine + nitroblue tetrazolium low activity Triticum aestivum ?
-
 ?
xanthine + 2,6-dichlorophenolindophenol + H2O
-
 Triticum aestivum urate + ?
-
 ?
xanthine + methyl viologen + H2O
-
 Triticum aestivum urate + reduced methyl viologen
-
 ?
xanthine + methylene blue + H2O 3fold higher activity compared to NAD+ as electron acceptor Triticum aestivum urate + reduced methylene blue
-
 ?
xanthine + NAD+ + H2O strict dehydrogenase activity, no utilization of O2 Triticum aestivum urate + NADH
-
 ?
xanthine + nitroblue tetrazolium + H2O
-
 Triticum aestivum urate + ?
-
 ?
xanthine + phenazine methosulfate + H2O 5fold higher activity compared to NAD+ as electron acceptor Triticum aestivum urate + ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.6 xanthine-NAD+, NADH-nitroblue tetrazolium or xanthine-nitroblue tetrazolium assay Triticum aestivum
9.8
-
 second optimum for NADH-nitroblue tetrazolium assay Triticum aestivum

Cofactor

Cofactor Comment Organism Structure
flavin
-
 Triticum aestivum
additional information
-
 Triticum aestivum
NAD+ cannot be replaced by NADP+ Triticum aestivum