Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Urate | 30% activation of hypoxanthine-NAD+ activity | Triticum aestivum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxymercuribenzoate | 76% inhibition of xanthine-NAD+-activity at 0.002 mM | Triticum aestivum | |
diethyldithiocarbamate | 72% inhibition of xanthine-NAD+-activity at 10 mM | Triticum aestivum | |
NaCN | 69% inhibition of xanthine-NAD+-activity at 3.3 mM | Triticum aestivum | |
o-phenanthroline | 12% inhibition of xanthine-NAD+-activity at 7.5 mM | Triticum aestivum | |
Salicylhydroxamic acid | 71% inhibition of xanthine-NAD+-activity at 5 mM | Triticum aestivum | |
Urate | 15% inhibition of xanthine-NAD+-activity | Triticum aestivum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0041 | - |
hypoxanthine | electron acceptor: NAD+ | Triticum aestivum | |
0.0056 | - |
phenazine methosulfate | electron donor: xanthine | Triticum aestivum | |
0.0057 | - |
NADH | electron acceptor: nitroblue tetrazolium | Triticum aestivum | |
0.0075 | - |
hypoxanthine | electron acceptor: nitroblue tetrazolium | Triticum aestivum | |
0.0082 | - |
xanthine | electron acceptor: NAD+ | Triticum aestivum | |
0.0086 | - |
xanthine | electron acceptor: nitroblue tetrazolium | Triticum aestivum | |
0.0113 | - |
nitroblue tetrazolium | electron donor: xanthine | Triticum aestivum | |
0.171 | - |
NAD+ | electron donor: xanthine | Triticum aestivum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
350000 | - |
native PAGE | Triticum aestivum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-4-hydroxy-pterin + NAD+ + H2O | Triticum aestivum | i.e. pterin | isoxanthopterin + NADH | - |
? | |
2-amino-4-hydroxy-pterin + NAD+ + H2O | Triticum aestivum | 11% of the activity compared to xanthine | isoxanthopterin + NADH | - |
? | |
purine + NAD+ + H2O | Triticum aestivum | poor substrate | ? + NADH | - |
? | |
xanthine + NAD+ + H2O | Triticum aestivum | strict dehydrogenase activity, no utilization of O2 | urate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Triticum aestivum | - |
wheat | - |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Triticum aestivum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Triticum aestivum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.23 | - |
xanthine-NAD+ assay | Triticum aestivum |
Storage Stability | Organism |
---|---|
4°C, 25 mM Tris-boric acid, pH 8.5, 2.5 mM EDTA, 2 mM 2-mercaptoethanol, 0.2 mM sodium molybdate, one year | Triticum aestivum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-4-hydroxy-pterin + NAD+ + H2O | i.e. pterin | Triticum aestivum | isoxanthopterin + NADH | - |
? | |
2-amino-4-hydroxy-pterin + NAD+ + H2O | 11% of the activity compared to xanthine | Triticum aestivum | isoxanthopterin + NADH | - |
? | |
2-amino-4-hydroxypterin + NAD+ + H2O | i.e. pterin | Triticum aestivum | isoxanthopterin + NADH | - |
? | |
2-amino-4-hydroxypterin + NAD+ + H2O | 11% of the activity compared to xanthine | Triticum aestivum | isoxanthopterin + NADH | - |
? | |
2-amino-4-hydroxypterin + nitroblue tetrazolium + H2O | very low activity | Triticum aestivum | isoxanthopterin + reduced nitroblue tetrazolium | - |
? | |
2-amino-4-hydroxypterin + nitroblue tetrazolium + H2O | i.e. pterin | Triticum aestivum | isoxanthopterin + reduced nitroblue tetrazolium | - |
? | |
4-hydroxypyrazolo(3,4-d)pyrimidine + nitroblue tetrazolium + H2O | very low activity | Triticum aestivum | 4,6-dihydroxypyrazolo(3,4-d)pyrimidine + reduced nitroblue tetrazolium | - |
? | |
4-hydroxypyrazolo(3,4-d)pyrimidine + nitroblue tetrazolium + H2O | i.e. allopurinol | Triticum aestivum | 4,6-dihydroxypyrazolo(3,4-d)pyrimidine + reduced nitroblue tetrazolium | - |
? | |
acetaldehyde + NAD+ + H2O | very low activity | Triticum aestivum | acetate + NADH + H+ | - |
? | |
acetaldehyde + nitroblue tetrazolium + H2O | very low activity | Triticum aestivum | acetic acid + reduced nitroblue tetrazolium | - |
? | |
benzaldehyde + nitroblue tetrazolium + H2O | very low activity | Triticum aestivum | benzoate + reduced nitroblue tetrazolium | - |
? | |
hypoxanthine + nitroblue tetrazolium | - |
Triticum aestivum | xanthine + ? | - |
? | |
NADH + electron acceptor + H2O | electron acceptor: nitroblue tetrazolium | Triticum aestivum | NAD+ + reduced electron acceptor | - |
? | |
NADPH + nitroblue tetrazolium + H2O | diaphorase activity | Triticum aestivum | NADP+ + reduced nitroblue tetrazolium | - |
? | |
purine + NAD+ + H2O | poor substrate | Triticum aestivum | ? + NADH | - |
? | |
purine + nitroblue tetrazolium | low activity | Triticum aestivum | ? | - |
? | |
xanthine + 2,6-dichlorophenolindophenol + H2O | - |
Triticum aestivum | urate + ? | - |
? | |
xanthine + methyl viologen + H2O | - |
Triticum aestivum | urate + reduced methyl viologen | - |
? | |
xanthine + methylene blue + H2O | 3fold higher activity compared to NAD+ as electron acceptor | Triticum aestivum | urate + reduced methylene blue | - |
? | |
xanthine + NAD+ + H2O | strict dehydrogenase activity, no utilization of O2 | Triticum aestivum | urate + NADH | - |
? | |
xanthine + nitroblue tetrazolium + H2O | - |
Triticum aestivum | urate + ? | - |
? | |
xanthine + phenazine methosulfate + H2O | 5fold higher activity compared to NAD+ as electron acceptor | Triticum aestivum | urate + ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.6 | xanthine-NAD+, NADH-nitroblue tetrazolium or xanthine-nitroblue tetrazolium assay | Triticum aestivum |
9.8 | - |
second optimum for NADH-nitroblue tetrazolium assay | Triticum aestivum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | - |
Triticum aestivum | |
additional information | - |
Triticum aestivum | |
NAD+ | cannot be replaced by NADP+ | Triticum aestivum |