BRENDA - Enzyme Database
show all sequences of 1.17.1.3

Transcripts of anthocyanidin reductase and leucoanthocyanidin reductase and measurement of catechin and epicatechin in tartary buckwheat

Kim, Y.B.; Thwe, A.A.; Kim, Y.; Li, X.; Cho, J.W.; Park, P.B.; Valan Arasu, M.; Abdullah Al-Dhabi, N.; Kim, S.J.; Suzuki, T.; Hyun Jho, K.; Park, S.U.; ScientificWorldJournal 2014, 726567 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene lar, DNA and amino acid sequence determination and analysis, sequence comaprisons and phylogenetic analysis, cloned from two cultivars, Hokkai T8 and T10, quantitative real-time RT-PCR enzyme expression analysis
Fagopyrum tataricum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Fagopyrum tataricum
5829
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+
Fagopyrum tataricum
-
(2R,3S)-catechin + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Fagopyrum tataricum
V9PJ26
cvs. Hokkai T8 and Hokkai T10
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
-
Fagopyrum tataricum
-
hairy root
-
Fagopyrum tataricum
-
leaf
-
Fagopyrum tataricum
-
additional information
gene expression of FtLAR during sprout development under dark and light conditions in cvs. T8 and T10, determination of catechin and epicatechin contents in sprouts, the expression pattern of anthocyanidin reductase (ANR) and leucoanthocyanidin reductase (LAR) do not match the accumulation pattern of proanthocyanidins in different organs of the two cultivars Hokkai T8 and Hokkai T10. FtLAR does not show large variations in its expression patterns among all the organs in cultivars Hokkai T8 and T10. Epicatechin content is higher than the catechin content in both T8 and T10. In T10, catechin is found mostly in the flowers and roots, whereas in T8, higher amounts are found in leaves and seeds at stage 1. Catechin content in T10 is highest in roots. Hokkai T10 wild roots in the soil and hairy roots are red in color. The catechin content is correlated with color pigment
Fagopyrum tataricum
-
root
-
Fagopyrum tataricum
-
seed
-
Fagopyrum tataricum
-
seedling
-
Fagopyrum tataricum
-
sprout
grown under light or dark condition
Fagopyrum tataricum
-
stem
-
Fagopyrum tataricum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+
-
746499
Fagopyrum tataricum
(2R,3S)-catechin + NADP+ + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 43300, about, sequence calculation
Fagopyrum tataricum
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Fagopyrum tataricum
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Fagopyrum tataricum
sequence calculation
-
5.27
Cloned(Commentary) (protein specific)
Commentary
Organism
gene lar, DNA and amino acid sequence determination and analysis, sequence comaprisons and phylogenetic analysis, cloned from two cultivars, Hokkai T8 and T10, quantitative real-time RT-PCR enzyme expression analysis
Fagopyrum tataricum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Fagopyrum tataricum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Fagopyrum tataricum
5829
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+
Fagopyrum tataricum
-
(2R,3S)-catechin + NADP+ + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
-
Fagopyrum tataricum
-
hairy root
-
Fagopyrum tataricum
-
leaf
-
Fagopyrum tataricum
-
additional information
gene expression of FtLAR during sprout development under dark and light conditions in cvs. T8 and T10, determination of catechin and epicatechin contents in sprouts, the expression pattern of anthocyanidin reductase (ANR) and leucoanthocyanidin reductase (LAR) do not match the accumulation pattern of proanthocyanidins in different organs of the two cultivars Hokkai T8 and Hokkai T10. FtLAR does not show large variations in its expression patterns among all the organs in cultivars Hokkai T8 and T10. Epicatechin content is higher than the catechin content in both T8 and T10. In T10, catechin is found mostly in the flowers and roots, whereas in T8, higher amounts are found in leaves and seeds at stage 1. Catechin content in T10 is highest in roots. Hokkai T10 wild roots in the soil and hairy roots are red in color. The catechin content is correlated with color pigment
Fagopyrum tataricum
-
root
-
Fagopyrum tataricum
-
seed
-
Fagopyrum tataricum
-
seedling
-
Fagopyrum tataricum
-
sprout
grown under light or dark condition
Fagopyrum tataricum
-
stem
-
Fagopyrum tataricum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+
-
746499
Fagopyrum tataricum
(2R,3S)-catechin + NADP+ + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 43300, about, sequence calculation
Fagopyrum tataricum
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Fagopyrum tataricum
sequence calculation
-
5.27
General Information
General Information
Commentary
Organism
metabolism
the enzyme is important in biosynthesis of proanthocyanidins (PAs) such as catechin and epicatechin, the proanthocyanidin pathway exists as ametabolic channel associated with cellular membranes
Fagopyrum tataricum
additional information
FtLAR had specific amino acid motifs of ICCN and THD
Fagopyrum tataricum
physiological function
leucoanthocyanidin reductase (LAR), together with anthocyanidin reductase (ANR, EC 1.3.1.77), plays an important role in the monomeric units biosynthesis of proanthocyanidins (PAs) such as catechin and epicatechin in several plants. ANR and LAR levels in tartary buckwheat might be regulated by different mechanisms for catechin and epicatechin biosynthesis under light and dark conditions. The catechin content is correlated with color pigment in roots
Fagopyrum tataricum
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is important in biosynthesis of proanthocyanidins (PAs) such as catechin and epicatechin, the proanthocyanidin pathway exists as ametabolic channel associated with cellular membranes
Fagopyrum tataricum
additional information
FtLAR had specific amino acid motifs of ICCN and THD
Fagopyrum tataricum
physiological function
leucoanthocyanidin reductase (LAR), together with anthocyanidin reductase (ANR, EC 1.3.1.77), plays an important role in the monomeric units biosynthesis of proanthocyanidins (PAs) such as catechin and epicatechin in several plants. ANR and LAR levels in tartary buckwheat might be regulated by different mechanisms for catechin and epicatechin biosynthesis under light and dark conditions. The catechin content is correlated with color pigment in roots
Fagopyrum tataricum
Other publictions for EC 1.17.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746163
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Evolutionary and functional c ...
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247
139-154
2018
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Metabolic characterization of ...
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205
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24
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1
3
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2
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2
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12
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14
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Gossypium hirsutum
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9
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1
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746499
Kim
Transcripts of anthocyanidin ...
Fagopyrum tataricum
ScientificWorldJournal
2014
726567
2014
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9
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3
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726337
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Isolation and characterization ...
Populus trichocarpa
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8
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2013
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727256
Liu
Proanthocyanidin synthesis in ...
Theobroma cacao
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746103
Pang
Mewan, K.M.; Sumner, L.W.; Yu ...
Camellia sinensis
Plant Physiol.
161
1103-1116
2013
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728054
Yuan
Molecular cloning and characte ...
Populus trichocarpa
J. Exp. Bot.
63
2513-2524
2012
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714393
Wang
-
Isolation and expression of ge ...
Diospyros kaki, Diospyros kaki Luotian-tianshi
Biol. Plant.
54
707-710
2010
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715907
Mauge
Crystal structure and catalyti ...
Vitis vinifera
J. Mol. Biol.
397
1079-1091
2010
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2
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1
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716027
Lacampagne
-
Involvement of abscisic acid i ...
Vitis vinifera
J. Plant Growth Regul.
29
81-90
2010
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1
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1
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1
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2
2
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700789
Gagne
Leucoanthocyanidin reductase a ...
Vitis vinifera
Plant Physiol. Biochem.
47
282-290
2009
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2
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6
1
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6
1
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676629
Paolocci
Ectopic expression of a basic ...
Lotus corniculatus
Plant Physiol.
143
504-516
2007
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1
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5
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1
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