BRENDA - Enzyme Database
show all sequences of 1.17.1.3

Pyramiding of tea dihydroflavonol reductase and anthocyanidin reductase increases flavan-3-ols and improves protective ability under stress conditions in tobacco

Kumar, V.; Yadav, S.K.; 3 Biotech 7, 177 (2017)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene LAR, co-overexpression with anthocyanidin reductase (ANR) in Nicotiana tabacum cv. Xanthi by Agrobacterium tumefaciens-mediated transformation, semiquantitative expression analysis
Camellia sinensis
Engineering
Protein Variants
Commentary
Organism
additional information
generation of pyramided transgenic lines overexpressing CsDFR and CsANR is implemented for evaluation purpose in relation to flavan-3-ol accumulation and for analyzing their combinatorial influence on the improvement of overall antioxidant potential, no morphological difference is observed between the CsDFR/CsANR overexpressing transgenic plants generated by reciprocal crosses with each other. No significant changes in growth pattern are observed in pyramided transgenic tobacco lines as compared to control tobacco plants. Both reciprocal crosses do not show significant change in plant height, stem diameter, and number of leaves as compared to control tobacco plants, which are measured at the flowering time, but the pyramided transgenic lines show a higher number of fruits/capsules per plant, increased seed yield per plant, and weight of seed (grams) compared to control tobacco plants. Increased transcript expression of flavan-3-ol/anthocyanin biosynthetic pathway genes in pyramided transgenic tobacco plants occurs. Improved antioxidant potential under the influence of CsDFR and CsANR overexpression in pyramided transgenic tobacco plants. Phenotype, overview
Camellia sinensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+
Camellia sinensis
-
(2R,3S)-catechin + NADP+ + H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Camellia sinensis
W6EL68
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Camellia sinensis
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+
-
743899
Camellia sinensis
(2R,3S)-catechin + NADP+ + H2O
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
LAR
-
Camellia sinensis
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Camellia sinensis
Cloned(Commentary) (protein specific)
Commentary
Organism
gene LAR, co-overexpression with anthocyanidin reductase (ANR) in Nicotiana tabacum cv. Xanthi by Agrobacterium tumefaciens-mediated transformation, semiquantitative expression analysis
Camellia sinensis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Camellia sinensis
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
generation of pyramided transgenic lines overexpressing CsDFR and CsANR is implemented for evaluation purpose in relation to flavan-3-ol accumulation and for analyzing their combinatorial influence on the improvement of overall antioxidant potential, no morphological difference is observed between the CsDFR/CsANR overexpressing transgenic plants generated by reciprocal crosses with each other. No significant changes in growth pattern are observed in pyramided transgenic tobacco lines as compared to control tobacco plants. Both reciprocal crosses do not show significant change in plant height, stem diameter, and number of leaves as compared to control tobacco plants, which are measured at the flowering time, but the pyramided transgenic lines show a higher number of fruits/capsules per plant, increased seed yield per plant, and weight of seed (grams) compared to control tobacco plants. Increased transcript expression of flavan-3-ol/anthocyanin biosynthetic pathway genes in pyramided transgenic tobacco plants occurs. Improved antioxidant potential under the influence of CsDFR and CsANR overexpression in pyramided transgenic tobacco plants. Phenotype, overview
Camellia sinensis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+
Camellia sinensis
-
(2R,3S)-catechin + NADP+ + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Camellia sinensis
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+
-
743899
Camellia sinensis
(2R,3S)-catechin + NADP+ + H2O
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in the flavan-3-ol/anthocyanin biosynthetic pathway. Leucoanthocyanidin reductase (LAR) and anthocyanidin reductase (ANR, EC 1.3.1.77) catalyze the formation of catechins and epicatechins from leucoanthocyanidins and anthocyanidins, respectively, overview
Camellia sinensis
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in the flavan-3-ol/anthocyanin biosynthetic pathway. Leucoanthocyanidin reductase (LAR) and anthocyanidin reductase (ANR, EC 1.3.1.77) catalyze the formation of catechins and epicatechins from leucoanthocyanidins and anthocyanidins, respectively, overview
Camellia sinensis
Other publictions for EC 1.17.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746163
Wang
Evolutionary and functional c ...
Camellia sinensis
Planta
247
139-154
2018
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3
9
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743899
Kumar
Pyramiding of tea dihydroflav ...
Camellia sinensis
3 Biotech
7
177
2017
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745825
Zhao
Metabolic characterization of ...
Camellia sinensis
Molecules
22
E2241
2017
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Matsui
Isolation and characterizatio ...
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J. Plant Physiol.
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Liu
A role for leucoanthocyanidin ...
Medicago truncatula, Medicago truncatula ecotype R108
Nat. Plants
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16182
2016
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2
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Nemesio-Gorriz
Different alleles of a gene e ...
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Plant Physiol.
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2671-2681
2016
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Liao
Molecular characterization of ...
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Front. Plant Sci.
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2015
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745035
Wen
Accumulation of flavanols and ...
Vitis vinifera
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2015
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728635
Xiao
Transcriptome and biochemical ...
Gossypium hirsutum
PLoS ONE
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2014
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746499
Kim
Transcripts of anthocyanidin ...
Fagopyrum tataricum
ScientificWorldJournal
2014
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2014
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726337
Wang
Isolation and characterization ...
Populus trichocarpa
PLoS ONE
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2013
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727256
Liu
Proanthocyanidin synthesis in ...
Theobroma cacao
BMC Plant Biol.
13
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2013
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746103
Pang
Mewan, K.M.; Sumner, L.W.; Yu ...
Camellia sinensis
Plant Physiol.
161
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2013
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728054
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Populus trichocarpa
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2012
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Diospyros kaki, Diospyros kaki Luotian-tianshi
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2010
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716027
Lacampagne
-
Involvement of abscisic acid i ...
Vitis vinifera
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29
81-90
2010
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2
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700789
Gagne
Leucoanthocyanidin reductase a ...
Vitis vinifera
Plant Physiol. Biochem.
47
282-290
2009
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676629
Paolocci
Ectopic expression of a basic ...
Lotus corniculatus
Plant Physiol.
143
504-516
2007
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676650
Pfeiffer
Biosynthesis of flavan 3-ols b ...
Malus domestica, Vitis vinifera
Plant Physiol. Biochem.
44
323-334
2006
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7
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15
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4
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676588
Bogs
Proanthocyanidin synthesis and ...
Vitis vinifera
Plant Physiol.
139
652-663
2005
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654366
Xie
Anthocyanidin reductases from ...
Arabidopsis thaliana, Medicago truncatula
Arch. Biochem. Biophys.
422
91-102
2004
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