Cloned (Comment) | Organism |
---|---|
gene LAR, co-overexpression with anthocyanidin reductase (ANR) in Nicotiana tabacum cv. Xanthi by Agrobacterium tumefaciens-mediated transformation, semiquantitative expression analysis | Camellia sinensis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of pyramided transgenic lines overexpressing CsDFR and CsANR is implemented for evaluation purpose in relation to flavan-3-ol accumulation and for analyzing their combinatorial influence on the improvement of overall antioxidant potential, no morphological difference is observed between the CsDFR/CsANR overexpressing transgenic plants generated by reciprocal crosses with each other. No significant changes in growth pattern are observed in pyramided transgenic tobacco lines as compared to control tobacco plants. Both reciprocal crosses do not show significant change in plant height, stem diameter, and number of leaves as compared to control tobacco plants, which are measured at the flowering time, but the pyramided transgenic lines show a higher number of fruits/capsules per plant, increased seed yield per plant, and weight of seed (grams) compared to control tobacco plants. Increased transcript expression of flavan-3-ol/anthocyanin biosynthetic pathway genes in pyramided transgenic tobacco plants occurs. Improved antioxidant potential under the influence of CsDFR and CsANR overexpression in pyramided transgenic tobacco plants. Phenotype, overview | Camellia sinensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+ | Camellia sinensis | - |
(2R,3S)-catechin + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Camellia sinensis | W6EL68 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Camellia sinensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+ | - |
Camellia sinensis | (2R,3S)-catechin + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LAR | - |
Camellia sinensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Camellia sinensis |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the flavan-3-ol/anthocyanin biosynthetic pathway. Leucoanthocyanidin reductase (LAR) and anthocyanidin reductase (ANR, EC 1.3.1.77) catalyze the formation of catechins and epicatechins from leucoanthocyanidins and anthocyanidins, respectively, overview | Camellia sinensis |