BRENDA - Enzyme Database show
show all sequences of 1.17.1.3

Isolation and expression of gene encoding leucoanthocyanidin reductase from Diospyros kaki during fruit development

Wang, Y.; Zhang, Q.; Luo, Z.; Biol. Plant. 54, 707-710 (2010)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene DkLAR, DNA and amino acid sequence determination and analysis, genotyping and phylogenetic analysis. Expression of the DkLAR gene in Chinese pollination-constant non-astringent, PCNA, genotype is coincident with the tannin cell development, but is not in Japanese PCNA and Chinese pollination-variant astringent PCA genotypes; gene DkLAR, DNA and amino acid sequence determination and analysis, genotyping. Expression of the DkLAR gene in Chinese pollination-constant non-astringent, PCNA, genotype is coincident with the tannin cell development, but is not in Japanese PCNA and Chinese pollination-variant astringent PCA genotypes
Diospyros kaki
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(2R,3S)-catechin + NADP+ + H2O
Diospyros kaki
-
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+
-
-
r
(2R,3S)-catechin + NADP+ + H2O
Diospyros kaki Luotian-tianshi
-
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Diospyros kaki
-
cv. of Chinese origin, gene DkLAR; cv. of Japanese origin, gene DkLAR
-
Diospyros kaki
E4W4T1
cv. of Chinese origin, gene DkLAR
-
Diospyros kaki Luotian-tianshi
-
cv. of Chinese origin, gene DkLAR; cv. of Japanese origin, gene DkLAR
-
Diospyros kaki Luotian-tianshi
E4W4T1
cv. of Chinese origin, gene DkLAR
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fruit
relationship of development of tannin cells and transcript level of the DkLAR gene, analysis of different cultivars, overview; relationship of development of tannin cells and transcript level of the DkLAR gene, analysis of different cultivars, overview
Diospyros kaki
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2R,3S)-catechin + NADP+ + H2O
-
714393
Diospyros kaki
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+
-
-
-
r
(2R,3S)-catechin + NADP+ + H2O
-
714393
Diospyros kaki Luotian-tianshi
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+
-
-
-
r
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
;
Diospyros kaki
Cloned(Commentary) (protein specific)
Commentary
Organism
gene DkLAR, DNA and amino acid sequence determination and analysis, genotyping and phylogenetic analysis. Expression of the DkLAR gene in Chinese pollination-constant non-astringent, PCNA, genotype is coincident with the tannin cell development, but is not in Japanese PCNA and Chinese pollination-variant astringent PCA genotypes
Diospyros kaki
gene DkLAR, DNA and amino acid sequence determination and analysis, genotyping. Expression of the DkLAR gene in Chinese pollination-constant non-astringent, PCNA, genotype is coincident with the tannin cell development, but is not in Japanese PCNA and Chinese pollination-variant astringent PCA genotypes
Diospyros kaki
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Diospyros kaki
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(2R,3S)-catechin + NADP+ + H2O
Diospyros kaki
-
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+
-
-
r
(2R,3S)-catechin + NADP+ + H2O
Diospyros kaki Luotian-tianshi
-
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+
-
-
r
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fruit
relationship of development of tannin cells and transcript level of the DkLAR gene, analysis of different cultivars, overview
Diospyros kaki
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2R,3S)-catechin + NADP+ + H2O
-
714393
Diospyros kaki
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+
-
-
-
r
(2R,3S)-catechin + NADP+ + H2O
-
714393
Diospyros kaki Luotian-tianshi
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+
-
-
-
r
General Information
General Information
Commentary
Organism
physiological function
leucoanthocyanidin reductase converts leucoanthocyanidin to (+)-catechin, a precursor of proanthocyanidins abundant in Japanese persimmon fruits; leucoanthocyanidin reductase converts leucoanthocyanidin to (+)-catechin, a precursor of proanthocyanidins abundant in Japanese persimmon fruits
Diospyros kaki
General Information (protein specific)
General Information
Commentary
Organism
physiological function
leucoanthocyanidin reductase converts leucoanthocyanidin to (+)-catechin, a precursor of proanthocyanidins abundant in Japanese persimmon fruits
Diospyros kaki
Other publictions for EC 1.17.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Fagopyrum tataricum
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9
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746103
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Camellia sinensis
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161
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1
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-
728054
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63
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-
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6
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714393
Wang
-
Isolation and expression of ge ...
Diospyros kaki, Diospyros kaki Luotian-tianshi
Biol. Plant.
54
707-710
2010
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715907
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397
1079-1091
2010
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1
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716027
Lacampagne
-
Involvement of abscisic acid i ...
Vitis vinifera
J. Plant Growth Regul.
29
81-90
2010
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2
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700789
Gagne
Leucoanthocyanidin reductase a ...
Vitis vinifera
Plant Physiol. Biochem.
47
282-290
2009
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676629
Paolocci
Ectopic expression of a basic ...
Lotus corniculatus
Plant Physiol.
143
504-516
2007
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676650
Pfeiffer
Biosynthesis of flavan 3-ols b ...
Malus domestica, Vitis vinifera
Plant Physiol. Biochem.
44
323-334
2006
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4
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676588
Bogs
Proanthocyanidin synthesis and ...
Vitis vinifera
Plant Physiol.
139
652-663
2005
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-
-
-
-
-
654366
Xie
Anthocyanidin reductases from ...
Arabidopsis thaliana, Medicago truncatula
Arch. Biochem. Biophys.
422
91-102
2004
-
-
2
-
-
-
8
2
-
1
-
4
-
4
-
-
2
-
-
-
-
-
12
-
2
-
-
-
2
-
-
3
-
-
-
-
-
2
3
-
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-
8
-
2
-
1
-
4
-
-
-
2
-
-
-
-
12
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
654372
Punyasiri
Flavonoid biosynthesis in the ...
Camellia sinensis
Arch. Biochem. Biophys.
431
22-30
2004
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
-
1
-
-
1
1
-
6
-
1
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2
-
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1
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1
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1
-
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1
-
1
1
-
6
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
636457
Tanner
Proanthocyanidin biosynthesis ...
Desmodium uncinatum
J. Biol. Chem.
278
31647-31656
2003
-
-
1
1
-
-
15
6
-
-
2
3
-
8
-
-
1
-
-
1
1
-
8
1
1
-
-
-
1
1
-
2
1
-
-
-
-
2
3
1
-
-
-
16
1
8
-
-
4
3
-
-
-
1
-
2
2
-
8
2
1
-
-
-
2
2
-
-
-
-
-
-
-
-
636459
Xie
Role of anthocyanidin reductas ...
Arabidopsis thaliana, Medicago truncatula
Science
299
396-399
2003
-
-
-
-
-
-
-
-
-
-
-
2
-
10
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
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-
2
-
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-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
636455
Joseph
-
Proanthocyanidin synthesis in ...
Onobrychis viciifolia
Aust. J. Plant Physiol.
25
271-278
1998
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
1
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2
-
-
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1
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-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
636458
Tanner
Synthesis of 3,4-cis[3H]leucoc ...
Hordeum vulgare, Onobrychis viciifolia
Anal. Biochem.
209
274-277
1993
-
-
-
-
-
2
-
1
-
-
-
2
-
2
-
-
-
-
-
2
-
2
4
-
-
-
-
-
2
2
-
4
-
-
-
-
-
-
4
-
-
2
-
-
-
1
-
-
-
2
-
-
-
-
-
2
-
2
4
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
347999
Stafford
Flavan-3-ol biosynthesis. The ...
Pseudotsuga menziesii
Plant Physiol.
76
184-186
1984
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
2
-
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3
-
-
-
-
-
1
1
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1
-
-
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-
1
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-
1
-
-
-
1
-
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2
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-
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1
1
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-