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Literature summary for 1.17.1.10 extracted from

  • Fogal, S.; Beneventi, E.; Cendron, L.; Bergantino, E.
    Structural basis for double cofactor specificity in a new formate dehydrogenase from the acidobacterium Granulicella mallensis MP5ACTX8 (2015), Appl. Microbiol. Biotechnol., 99, 9541-9554.
    View publication on PubMed
Search for more literature for 1.17.1.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Granulicella mallensis

Crystallization (Commentary)

Crystallization (Comment) Organism
apoprotein and holoprotein, either in complex with NAD+ or NADP+, hanging drop vapor diffusion method, using 0.1 M MES pH 6.5, 12% (w/v) PEG 2000 MME or 0. 1 M Na acetate pH 4.6, 2.0 M Na formate Granulicella mallensis

Inhibitors

Inhibitors Comment Organism Structure
DMSO 1-33% residual activity at 40% (v/v) Granulicella mallensis
ethanol complete inhibition at 40% (v/v) Granulicella mallensis
formaldehyde 79.8% residual activity at 0.1 M Granulicella mallensis
Isopropanol 33-66% residual activity at 20 and 40% (v/v) Granulicella mallensis
methanol complete inhibition at 40% (v/v) Granulicella mallensis
Sodium azide complete inhibition at 1 mM Granulicella mallensis
Sodium nitrate 44.3% residual activity at 0.1 M Granulicella mallensis
tert-butanol 33-66% residual activity at 10, 20 and 40% (v/v) Granulicella mallensis
Triton X-100 33-66% residual activity at 5 and 10% (v/v) Granulicella mallensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.85
-
 NADP+ at pH 7.0 and 30°C Granulicella mallensis
6.5
-
 NAD+ at pH 7.0 and 30°C Granulicella mallensis
80
-
 formate with NAD+ as cosubstrate, at pH 7.0 and 30°C Granulicella mallensis
200
-
 formate with NADP+ as cosubstrate, at pH 7.0 and 30°C Granulicella mallensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45200
-
 2 * 45200, SDS-PAGE Granulicella mallensis
79000
-
 gel filtration Granulicella mallensis

Organism

Organism UniProt Comment Textmining
Granulicella mallensis G8NVB5
-
-
Granulicella mallensis MP5ACTX8 G8NVB5
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, and Superdex 200 gel filtration Granulicella mallensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
 Granulicella mallensis CO2 + NADH + H+
-
 ?
formate + NAD+
-
 Granulicella mallensis MP5ACTX8 CO2 + NADH + H+
-
 ?
formate + NADP+ NADP+ is the preferred cofactor Granulicella mallensis CO2 + NADPH
-
 ?
formate + NADP+ NADP+ is the preferred cofactor Granulicella mallensis MP5ACTX8 CO2 + NADPH
-
 ?
additional information no activity is detected with malic acid, sodium citrate, sodium nitrate, succinic acid, sodium bicarbonate, sodium phosphite, ethanol, methanol, formaldehyde, sodium acetate, or sodium lactate Granulicella mallensis ?
-
 ?
additional information no activity is detected with malic acid, sodium citrate, sodium nitrate, succinic acid, sodium bicarbonate, sodium phosphite, ethanol, methanol, formaldehyde, sodium acetate, or sodium lactate Granulicella mallensis MP5ACTX8 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 45200, SDS-PAGE Granulicella mallensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 50 the enzyme remains stable after incubation for 25 h at 25°C and 37°C, while its activity is reduced to about 35% after 3 min at 50°C and is completely lost after 15 min at 50°C Granulicella mallensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.96
-
 NADP+ at pH 7.0 and 30°C Granulicella mallensis
5.77
-
 NAD+ at pH 7.0 and 30°C Granulicella mallensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 with NADP+ as cofactor Granulicella mallensis
7.5
-
 with NADP as cofactor Granulicella mallensis

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme shows double cofactor specificity, with NADP+ preferred over NAD+ at acidic pH values Granulicella mallensis
NADP+ the enzyme shows double cofactor specificity, with NADP+ preferred over NAD+ at acidic pH values Granulicella mallensis