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Literature summary for 1.17.1.10 extracted from
- Nicotinamide adenine dinucleotide phosphate-dependent formate dehydrogenase from Clostridium thermoaceticum: purification and properties (1974), J. Bacteriol., 120, 6-14.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sulfhydryl compounds | - |
Moorella thermoacetica |  |
General Stability
| General Stability | Organism |
|---|---|
| glycerol stabilizes during purification | Moorella thermoacetica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cyanide | - |
Moorella thermoacetica | |
| EDTA | - |
Moorella thermoacetica | |
| FeSO4 | - |
Moorella thermoacetica | |
| hypophosphite | inhibition removed by formate | Moorella thermoacetica | |
| mercaptoethanol | - |
Moorella thermoacetica | |
| NADP+ | substrate inhibition at 5 mM or higher concentration | Moorella thermoacetica | |
| Sodium selenite | 0.01 M | Moorella thermoacetica | |
| sulfite | inhibition of activity with NADP+ but not with methyl viologen | Moorella thermoacetica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.083 | - |
formate | - |
Moorella thermoacetica | |
| 0.11 | - |
NADP+ | - |
Moorella thermoacetica | |
| 0.277 | - |
formate | cosubstrate NADP+ | Moorella thermoacetica | |
| 2.35 | - |
reduced methyl viologen | - |
Moorella thermoacetica | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Moorella thermoacetica | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 270000 | 300000 | - |
Moorella thermoacetica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Moorella thermoacetica | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| extremely oxygen-sensitive | Moorella thermoacetica |
| rapid inactivation by air, thiol-iron complexes and formate stabilize | Moorella thermoacetica |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Moorella thermoacetica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 34 | - |
- |
Moorella thermoacetica |
| 113 | - |
methyl viologen as electron acceptor | Moorella thermoacetica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CO2 + NADPH | enzyme also catalyzes: 3. oxidation of NADPH with O2 | Moorella thermoacetica | formate + NADP+ | - |
r | |
| CO2 + NADPH | also reacts with: methyl viologen, benzyl viologen | Moorella thermoacetica | formate + NADP+ | - |
r | |
| formate + reduced methyl viologen | - |
Moorella thermoacetica | CO2 + oxidized methyl viologen | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
- |
Moorella thermoacetica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
1 min, absence of substrate, stable | Moorella thermoacetica |
| 73 | - |
10 min, 60% loss of activity | Moorella thermoacetica |
| 76 | - |
10 min, 90% loss of activity | Moorella thermoacetica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | - |
Moorella thermoacetica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Moorella thermoacetica | |
| NADPH | - |
Moorella thermoacetica | |
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