Literature summary for 1.16.3.4 extracted from

Roberts, S.; Wildner, G.; Grass, G.; Weichsel, A.; Ambrus, A.; Rensing, C.; Montfort, W.
A labile regulatory copper ion lies near the T1 copper site in the multicopper oxidase CueO (2003), J. Biol. Chem., 278, 31958-31963 .

Crystallization (Commentary)

Crystallization (Comment)	Organism
1.7 A resolution structure of a crystal soaked in CuCl2. A Cu(II) ion is bound to the protein 7.5 A from the T1 copper site in a region rich in methionine residues. The trigonal bipyramidal coordination sphere contains two methionine sulfur atoms, two aspartate carboxylate oxygen atoms, and a water molecule. Asp439 both ligates the labile copper and hydrogen-bonds to His443, which ligates the T1 copper. The type 3 copper atoms of the trinuclear cluster in the structure are bridged by a chloride ion that completes a square planar coordination sphere for the T2 copper atom	Escherichia coli

Crystallization (Comment)

Organism

1.7 A resolution structure of a crystal soaked in CuCl2. A Cu(II) ion is bound to the protein 7.5 A from the T1 copper site in a region rich in methionine residues. The trigonal bipyramidal coordination sphere contains two methionine sulfur atoms, two aspartate carboxylate oxygen atoms, and a water molecule. Asp439 both ligates the labile copper and hydrogen-bonds to His443, which ligates the T1 copper. The type 3 copper atoms of the trinuclear cluster in the structure are bridged by a chloride ion that completes a square planar coordination sphere for the T2 copper atom

Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
D360A	mutation in labile copper coordination sphere, inactive	Escherichia coli
D439A	mutation in labile copper coordination sphere, 3-4fold increase in Km value for Cu2+	Escherichia coli
M355L	mutation in labile copper coordination sphere, inactive	Escherichia coli
M441L	mutation in labile copper coordination sphere. Mutation has affected copper incorporation into the T1 copper site	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.163	-	Cu2+	wild-type, pH 6.5, 23°C	Escherichia coli
0.27	-	Cu2+	mutant M441L, pH 6.5, 23°C	Escherichia coli
0.59	-	Cu2+	mutant D439A, pH 6.5, 23°C	Escherichia coli
1.6	-	Cu2+	mutant D360A, pH 6.5, 23°C	Escherichia coli
82	-	Cu2+	mutant M355L, pH 6.5, 23°C	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P36649	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4 Cu2+ + 2 H2O	-	Escherichia coli	4 Cu+ + 4 H+ + O2	-	?

Expression

Organism	Comment	Expression
Escherichia coli	CueO is expressed under conditions of copper stress	up