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Literature summary for 1.16.3.3 extracted from

  • Su, J.; Bao, P.; Bai, T.; Deng, L.; Wu, H.; Liu, F.; He, J.
    CotA, a multicopper oxidase from Bacillus pumilus WH4, exhibits manganese-oxidase activity (2013), PLoS ONE, 8, e60573 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.16.3.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain M15 Bacillus pumilus

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 1.17% residual activity at 1.0 mM Bacillus pumilus
Ca2+ 0.83% residual activity at 1.0 mM Bacillus pumilus
dithiothreitol 54.75% residual activity at 1.0 mM Bacillus pumilus
EDTA 2.36% residual activity at 1.0 mM Bacillus pumilus
Guanidine-HCl 87.3% residual activity at 0.1 mM Bacillus pumilus
Hg2+ 0.77% residual activity at 1.0 mM Bacillus pumilus
Mg2+ 0.85% residual activity at 1.0 mM Bacillus pumilus
Ni2+ 0.76% residual activity at 1.0 mM Bacillus pumilus
o-phenanthroline 2.71% residual activity at 0.1 mM Bacillus pumilus
SDS 38.78% residual activity at 1% (w/v) Bacillus pumilus
Sr2+ 1.39% residual activity at 1.0 mM Bacillus pumilus
Zn2+ 0.46% residual activity at 1.0 mM Bacillus pumilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
14.85
-
 Mn2+ at pH 8.0 and 37°C Bacillus pumilus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ the enzyme contains approximately 3.5 copper atoms per molecule. 0.8 mM CuCl2 is used in assay conditions Bacillus pumilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Mn2+ + O2 + H2O Bacillus pumilus
-
 MnO2+ + H+
-
 ?
Mn2+ + O2 + H2O Bacillus pumilus WH4
-
 MnO2+ + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus pumilus I3XLC2
-
-
Bacillus pumilus WH4 I3XLC2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Bacillus pumilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Mn2+ + O2 + H2O
-
 Bacillus pumilus MnO2+ + H+
-
 ?
Mn2+ + O2 + H2O
-
 Bacillus pumilus WH4 MnO2+ + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Bacillus pumilus

Synonyms

Synonyms Comment Organism
CotA
-
 Bacillus pumilus
manganese-oxidase
-
 Bacillus pumilus
Mn(II) oxidase
-
 Bacillus pumilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
53
-
-
 Bacillus pumilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 60 more than 60% activity between 45 and 60°C Bacillus pumilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.32
-
 Mn2+ at pH 8.0 and 37°C Bacillus pumilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Bacillus pumilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.6 9.5 the enzyme remains stable after 24 h at pH 8.6-9.5 and shows about 50% residual activity after 24 h at pH 8.2 Bacillus pumilus