Cloned (Comment) | Organism |
---|---|
recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) by thermal treatment and two chromatographic steps | Campylobacter jejuni subsp. jejuni |
Protein Variants | Comment | Organism |
---|---|---|
H25G | site-directed mutagenesis, the mutant is able to accumulate iron in its inner cavity similar to the wild-type enzyme | Campylobacter jejuni subsp. jejuni |
H25G/H37G | site-directed mutagenesis, the mutant shows reduced activity to accumulate iron in its inner cavity compared to the wild-type enzyme | Campylobacter jejuni subsp. jejuni |
H37G | site-directed mutagenesis, the mutant is able to accumulate iron in its inner cavity similar to the wild-type enzyme | Campylobacter jejuni subsp. jejuni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic profile of wild-type and mutant enzymes, overview | Campylobacter jejuni subsp. jejuni |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | a non-heme iron protein | Campylobacter jejuni subsp. jejuni | |
Mn2+ | can bind to the enzyme, structure and kinetic analysis | Campylobacter jejuni subsp. jejuni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 Fe(II) + H2O2 + 2 H2O | Campylobacter jejuni subsp. jejuni | - |
2 [FeO(OH)] + 4 H+ | - |
? | |
2 Fe(II) + H2O2 + 2 H2O | Campylobacter jejuni subsp. jejuni ATCC 700819 | - |
2 [FeO(OH)] + 4 H+ | - |
? | |
2 Fe(II) + O2 + 4 H2O | Campylobacter jejuni subsp. jejuni | - |
2 [FeO(OH)] + 4 H+ + H2O2 | - |
? | |
2 Fe(II) + O2 + 4 H2O | Campylobacter jejuni subsp. jejuni ATCC 700819 | - |
2 [FeO(OH)] + 4 H+ + H2O2 | - |
? | |
4 Fe(II) + O2 + 6 H2O | Campylobacter jejuni subsp. jejuni | - |
4 [FeO(OH)] + 8 H+ | - |
? | |
4 Fe(II) + O2 + 6 H2O | Campylobacter jejuni subsp. jejuni ATCC 700819 | - |
4 [FeO(OH)] + 8 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni subsp. jejuni | Q0P891 | - |
- |
Campylobacter jejuni subsp. jejuni ATCC 700819 | Q0P891 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 Fe(II) + H2O2 + 2 H2O | - |
Campylobacter jejuni subsp. jejuni | 2 [FeO(OH)] + 4 H+ | - |
? | |
2 Fe(II) + H2O2 + 2 H2O | - |
Campylobacter jejuni subsp. jejuni ATCC 700819 | 2 [FeO(OH)] + 4 H+ | - |
? | |
2 Fe(II) + O2 + 4 H2O | - |
Campylobacter jejuni subsp. jejuni | 2 [FeO(OH)] + 4 H+ + H2O2 | - |
? | |
2 Fe(II) + O2 + 4 H2O | - |
Campylobacter jejuni subsp. jejuni ATCC 700819 | 2 [FeO(OH)] + 4 H+ + H2O2 | - |
? | |
4 Fe(II) + O2 + 6 H2O | - |
Campylobacter jejuni subsp. jejuni | 4 [FeO(OH)] + 8 H+ | - |
? | |
4 Fe(II) + O2 + 6 H2O | overall reaction | Campylobacter jejuni subsp. jejuni | 4 [FeO(OH)] + 8 H+ | - |
? | |
4 Fe(II) + O2 + 6 H2O | - |
Campylobacter jejuni subsp. jejuni ATCC 700819 | 4 [FeO(OH)] + 8 H+ | - |
? | |
4 Fe(II) + O2 + 6 H2O | overall reaction | Campylobacter jejuni subsp. jejuni ATCC 700819 | 4 [FeO(OH)] + 8 H+ | - |
? | |
additional information | the formation of the iron hydroxide core is monitored spectrophotometrically at 305 nm. The enzyme is able to bind DNA, DNA-binding analysis fro wild-type and mutant enzymes, overview | Campylobacter jejuni subsp. jejuni | ? | - |
? | |
additional information | the formation of the iron hydroxide core is monitored spectrophotometrically at 305 nm. The enzyme is able to bind DNA, DNA-binding analysis fro wild-type and mutant enzymes, overview | Campylobacter jejuni subsp. jejuni ATCC 700819 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | gel filtration and dynamic laser scaterring | Campylobacter jejuni subsp. jejuni |
Synonyms | Comment | Organism |
---|---|---|
CjDps | - |
Campylobacter jejuni subsp. jejuni |
DNA-binding protein from starved cells | - |
Campylobacter jejuni subsp. jejuni |
Dps protein | - |
Campylobacter jejuni subsp. jejuni |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 25 | assay at | Campylobacter jejuni subsp. jejuni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7 | assay at | Campylobacter jejuni subsp. jejuni |
General Information | Comment | Organism |
---|---|---|
evolution | Dps (DNA-binding protein from starved cells) belongs to a subfamily of ferritins. The presence of a ferroxidase centre, composed of highly conserved residues, is a signature of this protein family | Campylobacter jejuni subsp. jejuni |
additional information | two conserved histidine residues, H25 and H37, located at the ferroxidase centre of the Campylobacter jejuni Dps (DNA-binding protein from starved cells) protein, are not strictly required for metal binding and oxidation. The archetypical function of Dps seems to be DNA protection against hydroxyl radicals that are produced when Fe2+ and H2O2 combine | Campylobacter jejuni subsp. jejuni |
physiological function | Dps proteins are widely distributed in being required for survival during stressful conditions such as nutrient starvation, thermal stress and oxidative conditions, and inside biofilm. Campylobacter jejuni needs to be able to counteract various types of environmental stress during colonization, for example extreme pH and low availability of trace metals such as iron. Dps proteins can store iron atoms inside the dodecamer. Campylobacter jejuni Dps is able to bind DNA, and the DNA-binding activity is stimulated by Fe2+ (at room temperature in Bis-Tris, pH 6.0), overview | Campylobacter jejuni subsp. jejuni |