Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JRG2157 cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
microdialysis against 20 mM piperazine/HCl (pH 5.2) | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the non-heme enzyme contains 5-20 non-heme iron atoms per holomer | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19400 | - |
24 * 19400, SDS-PAGE | Escherichia coli |
19424 | - |
24 * 19424, calculated from amino acid sequence | Escherichia coli |
465000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fe(II) + O2 + H2O | Escherichia coli | - |
[FeO(OH)] + H+ + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A998 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Q-Sepharose column chromatography, and Sephacryl S300 gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fe(II) + O2 + H2O | - |
Escherichia coli | [FeO(OH)] + H+ + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
24-mer | 24 * 19400, SDS-PAGE | Escherichia coli |
24-mer | 24 * 19424, calculated from amino acid sequence | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
ferritin | - |
Escherichia coli |
Ftn | - |
Escherichia coli |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Escherichia coli | isoelectric focusing | - |
5 |