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Literature summary for 1.16.1.8 extracted from

  • Haque, M.M.; Bayachou, M.; Tejero, J.; Kenney, C.T.; Pearl, N.M.; Im, S.C.; Waskell, L.; Stuehr, D.J.
    Distinct conformational behaviors of four mammalian dual-flavin reductases (cytochrome P450 reductase, methionine synthase reductase, neuronal nitric oxide synthase, endothelial nitric oxide synthase) determine their unique catalytic profiles (2014), FEBS J., 281, 5325-5340 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information stopped-flow spectroscopy, single turnover methods and a kinetic model relating electron flux through the enzyme to its conformational setpoint and its rates of conformational switching, kinetic model for electron flux through a dual-flavin enzyme, overview Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 [methionine synthase]-cob(II)alamin + NADPH + H+ + 2 S-adenosyl-L-methionine Homo sapiens
-
2 [methionine synthase]-methylcob(I)alamin + 2 S-adenosylhomocysteine + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UBK8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 [methionine synthase]-cob(II)alamin + NADPH + H+ + 2 S-adenosyl-L-methionine
-
Homo sapiens 2 [methionine synthase]-methylcob(I)alamin + 2 S-adenosylhomocysteine + NADP+
-
?
additional information fully reduced enzyme reaction with excess of cytochrome c, kinetics, overview. The NADPH-bound, fully reduced MSR completes most of its first turnover within the mixing dead time of the instrument, leaving only approximately 28% of the first turnover observable Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
More structure-based analysis of the domain interfaces Homo sapiens

Synonyms

Synonyms Comment Organism
Methionine synthase reductase
-
Homo sapiens
MSR
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD a dual-flavin reductase Homo sapiens
FMN a dual-flavin reductase Homo sapiens
additional information structure-based analysis of the cofactor domain interfaces, overview Homo sapiens
NADPH
-
Homo sapiens