BRENDA - Enzyme Database show
show all sequences of 1.16.1.7

Sequence diversity and enzyme activity of ferric-chelate reductase LeFRO1 in tomato

Kong, D.; Chen, C.; Wu, H.; Li, Y.; Li, J.; Ling, H.Q.; J. Genet. Genomics 40, 565-573 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene LeFRO1, DNA and amino acid sequence determination and analysis from 16 different cultivars, genotyping detects three types of LeFRO1 (LeFRO1MM, LeFRO1Ailsa and LeFRO1Monita) with five amino acid variations at the positions 21, 24, 112, 195 and 582
Solanum lycopersicum
Engineering
Amino acid exchange
Commentary
Organism
A112X
modification of this amino acid results in a significant reduction of enzyme activity
Solanum lycopersicum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 Fe(II)-siderophore + NAD+ + H+
Solanum lycopersicum
-
2 Fe(III)-siderophore + NADH
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Solanum lycopersicum
-
16 cultivars
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
-
Solanum lycopersicum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 Fe(II)-siderophore + NAD+ + H+
-
728066
Solanum lycopersicum
2 Fe(III)-siderophore + NADH
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Solanum lycopersicum
Cloned(Commentary) (protein specific)
Commentary
Organism
gene LeFRO1, DNA and amino acid sequence determination and analysis from 16 different cultivars, genotyping detects three types of LeFRO1 (LeFRO1MM, LeFRO1Ailsa and LeFRO1Monita) with five amino acid variations at the positions 21, 24, 112, 195 and 582
Solanum lycopersicum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Solanum lycopersicum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
A112X
modification of this amino acid results in a significant reduction of enzyme activity
Solanum lycopersicum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 Fe(II)-siderophore + NAD+ + H+
Solanum lycopersicum
-
2 Fe(III)-siderophore + NADH
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
-
Solanum lycopersicum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 Fe(II)-siderophore + NAD+ + H+
-
728066
Solanum lycopersicum
2 Fe(III)-siderophore + NADH
-
-
-
?
General Information
General Information
Commentary
Organism
additional information
Ala112 of LeFRO1 is critical for maintaining the high activity of ferric-chelate reductase, modification of this amino acid results in a significant reduction of enzyme activity. The combination of the amino acid residue Ile at the site 24 with Lys at the site 582 plays a positive role in the enzyme activity of LeFRO1, genotyping, overview
Solanum lycopersicum
physiological function
ferric-chelate reductase which functions in the reduction of ferric to ferrous iron on root surface is a critical protein for iron homeostasis in strategy I plants. LeFRO1 is a major ferric-chelate reductase involved in iron uptake in tomato
Solanum lycopersicum
General Information (protein specific)
General Information
Commentary
Organism
additional information
Ala112 of LeFRO1 is critical for maintaining the high activity of ferric-chelate reductase, modification of this amino acid results in a significant reduction of enzyme activity. The combination of the amino acid residue Ile at the site 24 with Lys at the site 582 plays a positive role in the enzyme activity of LeFRO1, genotyping, overview
Solanum lycopersicum
physiological function
ferric-chelate reductase which functions in the reduction of ferric to ferrous iron on root surface is a critical protein for iron homeostasis in strategy I plants. LeFRO1 is a major ferric-chelate reductase involved in iron uptake in tomato
Solanum lycopersicum
Other publictions for EC 1.16.1.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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728066
Kong
Sequence diversity and enzyme ...
Solanum lycopersicum
J. Genet. Genomics
40
565-573
2013
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Root ferric chelate reductase ...
Fragaria sp.
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Reduction of Fe(III)EDTA in a ...
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Pestana
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The root ferric-chelate reduct ...
Ceratonia siliqua, Citrus trifoliata
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135
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2012
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2
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716576
Mikami
Allocation of Fe and ferric ch ...
Hordeum vulgare, Hordeum vulgare Ehimehadaka No. 1, Sorghum bicolor, Sorghum bicolor Keller
Plant Physiol. Biochem.
49
513-519
2011
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716177
Li
Overexpression of AtFRO6 in tr ...
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2010
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Chen
Nitric oxide acts downstream o ...
Arabidopsis thaliana
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2010
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Arachis hypogaea, Arachis hypogaea Nongda 818
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689790
Jeong
Chloroplast Fe(III) chelate re ...
Arabidopsis thaliana, Saccharomyces cerevisiae
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105
10619-10624
2008
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1
2
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690052
He
Use of the modified viral sate ...
Solanum lycopersicum
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51
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2008
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699397
Jin
Iron deficiency-induced increa ...
Trifolium pratense
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50
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2008
1
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Ishimaru
Mutational reconstructed ferri ...
Oryza sativa
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104
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1
1
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676514
Durrett
Arabidopsis cpFtsY mutants exh ...
Arabidopsis thaliana
Plant J.
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2006
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676551
Schagerloef
Transmembrane topology of FRO2 ...
Arabidopsis thaliana
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Arabidopsis thaliana
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140
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676697
Mukherjee
Expression profiling of the Ar ...
Arabidopsis thaliana
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223
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2006
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676704
Vasconcelos
Molecular and phenotypic chara ...
Arabidopsis thaliana
Planta
224
1116-1128
2006
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659789
Molassiotis
-
Induction of Fe(III)-chelate r ...
Prunus cerasifera, Prunus dulcis, Prunus persica
J. Plant Nutr.
28
669-682
2005
3
3
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676455
Wu
Molecular and biochemical char ...
Arabidopsis thaliana
Plant Cell Physiol.
46
1505-1514
2005
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657543
Poonnachit
Effect of ammonium and nitrate ...
Vaccinium arboreum, Vaccinium corymbosum
Ann. Bot.
93
399-405
2004
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2
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659788
Ojeda
-
Root and leaf ferric chelate r ...
Annona glabra, Annona muricata
J. Plant Nutr.
27
1381-1393
2004
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2
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660081
Kosegarten
-
Apoplastic pH and FeIII reduct ...
Helianthus annuus
Physiol. Plant.
122
95-106
2004
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1
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