BRENDA - Enzyme Database show
show all sequences of 1.16.1.7

Chloroplast Fe(III) chelate reductase activity is essential for seedling viability under iron limiting conditions

Jeong, J.; Cohu, C.; Kerkeb, L.; Pilon, M.; Connolly, E.L.; Guerinot, M.L.; Proc. Natl. Acad. Sci. USA 105, 10619-10624 (2008)

Data extracted from this reference:

Application
Application
Commentary
Organism
agriculture
ferric reductase oxidase 7 is a chloroplast Fe(III) chelate reductase required for survival under ironlimiting conditions, for efficient photosynthesis, and for proper chloroplast iron acquisition in young seedlings
Arabidopsis thaliana
Cloned(Commentary)
Commentary
Organism
expression of ferric reductase oxidase FRO6-GFP or FRO7-GFP in yeast
Saccharomyces cerevisiae
transient expression of yeast ferric reductase oxidase FRO6-GFP or FRO7-GFP in Arabidopsis protoplasts, generation of transgenic plants expressing glucuronidase reporter gene fused to the FRO7 promoter, generation of Fro7 mutants missing the Fe(III) chelate reductase
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
membrane
Arabidopsis thaliana
9507
-
mitochondrion
FRO7
Saccharomyces cerevisiae
5739
-
plasma membrane
FRO6
Saccharomyces cerevisiae
5886
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
ferric reductase oxidase 7 is a chloroplast Fe(III) chelate reductase required for survival under ironlimiting conditions, for efficient photosynthesis, and for proper chloroplast iron acquisition in young seedlings
Arabidopsis thaliana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
wild type and transgenic plants expressing a yeast ferric chelate reductase gene, mutants missing the Fe(III) chelate reductase
-
Saccharomyces cerevisiae
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
plant
-
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 Fe(II) + NAD+
chloroplats with 300 micromolar ferrozine and 100 micromolar Fe-EDTA in Hepes-sorbitol buffer, pH 7.3
689790
Arabidopsis thaliana
2 Fe(III) + NADH + H+
-
-
-
?
2 Fe2+ + NAD+
transgenic FRO6 yeast has higher Fe3+ chelate reductase activity, Fe3+ chelate reductase activity of FRO7-expressing cells does not differ from the basal level observed in the vector-only control cells
689790
Saccharomyces cerevisiae
2 Fe3+ + NADH + H+
-
-
-
?
Application (protein specific)
Application
Commentary
Organism
agriculture
ferric reductase oxidase 7 is a chloroplast Fe(III) chelate reductase required for survival under ironlimiting conditions, for efficient photosynthesis, and for proper chloroplast iron acquisition in young seedlings
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
transient expression of yeast ferric reductase oxidase FRO6-GFP or FRO7-GFP in Arabidopsis protoplasts, generation of transgenic plants expressing glucuronidase reporter gene fused to the FRO7 promoter, generation of Fro7 mutants missing the Fe(III) chelate reductase
Arabidopsis thaliana
expression of ferric reductase oxidase FRO6-GFP or FRO7-GFP in yeast
Saccharomyces cerevisiae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
membrane
Arabidopsis thaliana
9507
-
mitochondrion
FRO7
Saccharomyces cerevisiae
5739
-
plasma membrane
FRO6
Saccharomyces cerevisiae
5886
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
ferric reductase oxidase 7 is a chloroplast Fe(III) chelate reductase required for survival under ironlimiting conditions, for efficient photosynthesis, and for proper chloroplast iron acquisition in young seedlings
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
plant
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 Fe(II) + NAD+
chloroplats with 300 micromolar ferrozine and 100 micromolar Fe-EDTA in Hepes-sorbitol buffer, pH 7.3
689790
Arabidopsis thaliana
2 Fe(III) + NADH + H+
-
-
-
?
2 Fe2+ + NAD+
transgenic FRO6 yeast has higher Fe3+ chelate reductase activity, Fe3+ chelate reductase activity of FRO7-expressing cells does not differ from the basal level observed in the vector-only control cells
689790
Saccharomyces cerevisiae
2 Fe3+ + NADH + H+
-
-
-
?
Other publictions for EC 1.16.1.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745903
Siso-Terraza
Flavins secreted by roots of ...
Beta vulgaris
New Phytol.
209
733-745
2016
2
-
-
-
-
-
1
-
-
-
-
1
-
3
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
2
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
728066
Kong
Sequence diversity and enzyme ...
Solanum lycopersicum
J. Genet. Genomics
40
565-573
2013
-
-
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
728202
Pestana
-
Root ferric chelate reductase ...
Fragaria sp.
J. Plant Nutr.
36
2035-2047
2013
-
-
1
-
-
-
-
-
-
2
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-
727183
Dong
Reduction of Fe(III)EDTA in a ...
Paracoccus denitrificans, Paracoccus denitrificans ZGL1
Biores. Technol.
120
127-132
2012
-
-
-
-
-
-
2
-
3
1
-
2
-
4
-
-
-
-
-
-
2
-
4
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
3
1
-
2
-
-
-
-
-
-
2
-
4
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
728795
Pestana
-
The root ferric-chelate reduct ...
Ceratonia siliqua, Citrus trifoliata
Sci. Hortic.
135
65-67
2012
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
-
2
2
2
2
-
-
716576
Mikami
Allocation of Fe and ferric ch ...
Hordeum vulgare, Hordeum vulgare Ehimehadaka No. 1, Sorghum bicolor, Sorghum bicolor Keller
Plant Physiol. Biochem.
49
513-519
2011
-
-
2
-
-
-
-
-
4
-
-
-
-
8
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
1
2
3
1
-
-
716177
Li
Overexpression of AtFRO6 in tr ...
Arabidopsis thaliana
Mol. Biol. Rep.
38
3605-3616
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
4
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
716609
Chen
Nitric oxide acts downstream o ...
Arabidopsis thaliana
Plant Physiol.
154
810-819
2010
-
-
-
-
-
-
-
-
2
-
-
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
700646
Ding
Regulation of AhFRO1, an Fe(II ...
Arachis hypogaea, Arachis hypogaea Nongda 818
Physiol. Plant.
136
274-283
2009
-
-
1
-
-
-
-
-
1
-
-
-
-
5
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
689790
Jeong
Chloroplast Fe(III) chelate re ...
Arabidopsis thaliana, Saccharomyces cerevisiae
Proc. Natl. Acad. Sci. USA
105
10619-10624
2008
-
1
2
-
-
-
-
-
3
1
-
-
-
3
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690052
He
Use of the modified viral sate ...
Solanum lycopersicum
Sci. China C Life Sci.
51
402-409
2008
-
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
699397
Jin
Iron deficiency-induced increa ...
Trifolium pratense
J. Integr. Plant Biol.
50
1557-1562
2008
1
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689782
Ishimaru
Mutational reconstructed ferri ...
Oryza sativa
Proc. Natl. Acad. Sci. USA
104
7373-7378
2007
-
1
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676514
Durrett
Arabidopsis cpFtsY mutants exh ...
Arabidopsis thaliana
Plant J.
47
467-479
2006
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676551
Schagerloef
Transmembrane topology of FRO2 ...
Arabidopsis thaliana
Plant Mol. Biol.
62
215-221
2006
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676592
Feng
Light-regulated, tissue-specif ...
Arabidopsis thaliana
Plant Physiol.
140
1345-1354
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676697
Mukherjee
Expression profiling of the Ar ...
Arabidopsis thaliana
Planta
223
1178-1190
2006
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676704
Vasconcelos
Molecular and phenotypic chara ...
Arabidopsis thaliana
Planta
224
1116-1128
2006
-
1
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659789
Molassiotis
-
Induction of Fe(III)-chelate r ...
Prunus cerasifera, Prunus dulcis, Prunus persica
J. Plant Nutr.
28
669-682
2005
3
3
-
-
-
-
4
-
-
3
-
-
-
3
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
-
-
-
4
-
-
-
3
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676455
Wu
Molecular and biochemical char ...
Arabidopsis thaliana
Plant Cell Physiol.
46
1505-1514
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657543
Poonnachit
Effect of ammonium and nitrate ...
Vaccinium arboreum, Vaccinium corymbosum
Ann. Bot.
93
399-405
2004
-
2
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659788
Ojeda
-
Root and leaf ferric chelate r ...
Annona glabra, Annona muricata
J. Plant Nutr.
27
1381-1393
2004
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660081
Kosegarten
-
Apoplastic pH and FeIII reduct ...
Helianthus annuus
Physiol. Plant.
122
95-106
2004
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
660227
Connolly
Overexpression of the FRO2 fer ...
Arabidopsis thaliana
Plant Physiol.
133
1102-1110
2003
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660269
Chang
-
Effects of cadmium and lead on ...
Beta vulgaris
Plant Physiol. Biochem.
41
999-1005
2003
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438036
Schmidt
-
Orientation of NADH-linked fer ...
Plantago lanceolata
Protoplasma
203
186-193
1998
2
-
-
-
-
-
1
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438035
Susin
The pH requirement for in vivo ...
Beta vulgaris
Plant Physiol.
110
111-123
1996
-
-
-
-
-
-
-
1
1
-
-
-
-
3
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
438034
Holden
Fe3+-chelate reductase activit ...
Solanum lycopersicum, Solanum lycopersicum Mill.
Plant Physiol.
97
537-544
1991
-
-
-
-
-
-
-
1
1
-
-
-
-
10
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-