Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.16.1.1 extracted from

  • Bafana, A.; Khan, F.; Suguna, K.
    Structural and functional characterization of mercuric reductase from Lysinibacillus sphaericus strain G1 (2017), Biometals, 30, 809-819 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-meracptoethanol activates at 0.5-1.0 mM Lysinibacillus sphaericus

Application

Application Comment Organism
environmental protection enzyme MerA is a promising candidate for Hg2+ bioremediation Lysinibacillus sphaericus

Cloned(Commentary)

Cloned (Comment) Organism
gene merA, overexpression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Lysinibacillus sphaericus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His-tagged enzyme, mixing of 10 mg/ml protein in 50 mM phosphate buffer, pH 7.2, with reservoir solution containing 5% v/v Tascimate, 0.1 M HEPES, pH 7.0, and 10% w/v PEG monomethyl ether 5000, X-ray diffraction structure determination and analysis at 3.48 A resolution Lysinibacillus sphaericus

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 60% inhibition at 0.1 mM Lysinibacillus sphaericus
Co2+ 40% inhibition at 0.1 mM Lysinibacillus sphaericus
Cu2+ 80% inhibition at 0.1 mM, mixed inhibition pattern Lysinibacillus sphaericus
Mg2+ 15% inhibition at 0.1 mM Lysinibacillus sphaericus
Zn2+ 45% inhibition at 0.1 mM Lysinibacillus sphaericus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Lysinibacillus sphaericus
0.032
-
Hg2+ pH 7.4, 25°C, recombinant enzyme Lysinibacillus sphaericus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information 0.1 mM of Cr6+ and Fe3+ do not have significant effect on the enzyme activity Lysinibacillus sphaericus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
recombinant enzyme, gel filtration Lysinibacillus sphaericus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Hg2+ + NADPH Lysinibacillus sphaericus
-
Hg + NADP+ + H+
-
?
Hg2+ + NADPH Lysinibacillus sphaericus G1
-
Hg + NADP+ + H+
-
?

Organism

Organism UniProt Comment Textmining
Lysinibacillus sphaericus D9J041
-
-
Lysinibacillus sphaericus G1 D9J041
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, Lysinibacillus sphaericus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Hg2+ + NADPH
-
Lysinibacillus sphaericus Hg + NADP+ + H+
-
?
Hg2+ + NADPH
-
Lysinibacillus sphaericus G1 Hg + NADP+ + H+
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 60000, recombinant His-tagged enzyme, SDS-PAGE Lysinibacillus sphaericus

Synonyms

Synonyms Comment Organism
MerA
-
Lysinibacillus sphaericus
mercuric reductase
-
Lysinibacillus sphaericus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 50 broad optimum Lysinibacillus sphaericus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 50 purified recombinant enzyme, completely stable at Lysinibacillus sphaericus
60 70 purified recombinant enzyme, slight loss of activity 60°C, loss of 90% activity at 70°C and above Lysinibacillus sphaericus
67
-
Tm of enzyme MerA Lysinibacillus sphaericus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17.7
-
Hg2+ pH 7.4, 25°C, recombinant enzyme Lysinibacillus sphaericus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
Lysinibacillus sphaericus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 activity range, profile overview, inactive at pH 5.0 Lysinibacillus sphaericus

Cofactor

Cofactor Comment Organism Structure
FAD the prosthetic group Lysinibacillus sphaericus
NADPH preferred electron donor Lysinibacillus sphaericus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
553
-
Hg2+ pH 7.4, 25°C, recombinant enzyme Lysinibacillus sphaericus