Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-meracptoethanol | activates at 0.5-1.0 mM | Lysinibacillus sphaericus |
Application | Comment | Organism |
---|---|---|
environmental protection | enzyme MerA is a promising candidate for Hg2+ bioremediation | Lysinibacillus sphaericus |
Cloned (Comment) | Organism |
---|---|
gene merA, overexpression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Lysinibacillus sphaericus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, mixing of 10 mg/ml protein in 50 mM phosphate buffer, pH 7.2, with reservoir solution containing 5% v/v Tascimate, 0.1 M HEPES, pH 7.0, and 10% w/v PEG monomethyl ether 5000, X-ray diffraction structure determination and analysis at 3.48 A resolution | Lysinibacillus sphaericus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 60% inhibition at 0.1 mM | Lysinibacillus sphaericus | |
Co2+ | 40% inhibition at 0.1 mM | Lysinibacillus sphaericus | |
Cu2+ | 80% inhibition at 0.1 mM, mixed inhibition pattern | Lysinibacillus sphaericus | |
Mg2+ | 15% inhibition at 0.1 mM | Lysinibacillus sphaericus | |
Zn2+ | 45% inhibition at 0.1 mM | Lysinibacillus sphaericus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Lysinibacillus sphaericus | |
0.032 | - |
Hg2+ | pH 7.4, 25°C, recombinant enzyme | Lysinibacillus sphaericus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | 0.1 mM of Cr6+ and Fe3+ do not have significant effect on the enzyme activity | Lysinibacillus sphaericus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
recombinant enzyme, gel filtration | Lysinibacillus sphaericus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Hg2+ + NADPH | Lysinibacillus sphaericus | - |
Hg + NADP+ + H+ | - |
? | |
Hg2+ + NADPH | Lysinibacillus sphaericus G1 | - |
Hg + NADP+ + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus sphaericus | D9J041 | - |
- |
Lysinibacillus sphaericus G1 | D9J041 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, | Lysinibacillus sphaericus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Hg2+ + NADPH | - |
Lysinibacillus sphaericus | Hg + NADP+ + H+ | - |
? | |
Hg2+ + NADPH | - |
Lysinibacillus sphaericus G1 | Hg + NADP+ + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 60000, recombinant His-tagged enzyme, SDS-PAGE | Lysinibacillus sphaericus |
Synonyms | Comment | Organism |
---|---|---|
MerA | - |
Lysinibacillus sphaericus |
mercuric reductase | - |
Lysinibacillus sphaericus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | broad optimum | Lysinibacillus sphaericus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | purified recombinant enzyme, completely stable at | Lysinibacillus sphaericus |
60 | 70 | purified recombinant enzyme, slight loss of activity 60°C, loss of 90% activity at 70°C and above | Lysinibacillus sphaericus |
67 | - |
Tm of enzyme MerA | Lysinibacillus sphaericus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17.7 | - |
Hg2+ | pH 7.4, 25°C, recombinant enzyme | Lysinibacillus sphaericus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Lysinibacillus sphaericus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | activity range, profile overview, inactive at pH 5.0 | Lysinibacillus sphaericus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the prosthetic group | Lysinibacillus sphaericus | |
NADPH | preferred electron donor | Lysinibacillus sphaericus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
553 | - |
Hg2+ | pH 7.4, 25°C, recombinant enzyme | Lysinibacillus sphaericus |