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Literature summary for 1.16.1.1 extracted from

  • Ledwidge, R.; Patel, B.; Dong, A.; Fiedler, D.; Falkowski, M.; Zelikova, J.; Summers, A.O.; Pai, E.F.; Miller, S.M.
    NmerA, the metal binding domain of mercuric ion reductase, removes Hg2+ from proteins, delivers it to the catalytic core, and protects cells under glutathione-depleted conditions (2005), Biochemistry, 44, 11402-11416.
    View publication on PubMed
Search for more literature for 1.16.1.1

Protein Variants

Protein Variants Comment Organism
additional information cloning and expression of catalytic core and N-terminal domain of enzyme as separate proteins. the N-terminal domain NmerA is a stable, soluble protein that binds 1 Hg2+ per domain and delivers it to the catalytic core at kinetically competent rates Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 Hg2+ recombinant catalytic core, presence of N-terminal sequence NmerA, 25°C, pH 7.3 Bacillus sp. (in: Bacteria)
0.297
-
 Hg2+ full-length enzyme, 25°C, pH 7.3 Bacillus sp. (in: Bacteria)
1.177
-
 Hg2+ recombinant catalytic core, 25°C, pH 7.3 Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) RC607
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Hg2+ + NADPH
-
 Bacillus sp. (in: Bacteria) Hg + NADP+ + H+
-
 ?
Hg2+ + NADPH
-
 Bacillus sp. (in: Bacteria) RC607 Hg + NADP+ + H+
-
 ?

Subunits

Subunits Comment Organism
More N-terminal domain NmerA participates in acquisition and delivery of Hg2+ to the catalytic core during thr reduction catalyzed by full-length enzyme Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7
-
 Hg2+ recombinant catalytic core, 25°C, pH 7.3 Bacillus sp. (in: Bacteria)
9
-
 Hg2+ full-length enzyme, 25°C, pH 7.3 Bacillus sp. (in: Bacteria)
12
-
 Hg2+ recombinant catalytic core, presence of N-terminal sequence NmerA, 25°C, pH 7.3 Bacillus sp. (in: Bacteria)