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Literature summary for 1.16.1.1 extracted from

  • Ghosh, S.; Sadhukhan, P.C.; Chaudhuri, J.; Ghosh, D.K.; Mandal, A.
    Purification and properties of mercuric reductase from Azotobacter chroococcum (1999), J. Appl. Microbiol., 86, 7-12.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol required Azotobacter chroococcum
2-mercaptoethanol optimal concentration: 2 mM Azotobacter chroococcum

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 0.1 mM Azotobacter chroococcum
Bi3+ 0.1 mM, 40% inhibition Azotobacter chroococcum
Cd2+
-
Azotobacter chroococcum
CoCl2
-
Azotobacter chroococcum
Cu2+ 0.1 mM, 44% inhibition Azotobacter chroococcum
HgCl2 activity is inhibited by an excess of HgCl2 Azotobacter chroococcum
NaN3
-
Azotobacter chroococcum
NEM 2 mM, 60% inhibition Azotobacter chroococcum
Ni2+ 0.1 mM, 62% inhibition Azotobacter chroococcum
Pb2+ 0.1 mM, 60% inhibition Azotobacter chroococcum
Zn2+ 0.1 mM, 60% inhibition Azotobacter chroococcum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
Hg
-
Azotobacter chroococcum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Azotobacter chroococcum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
1 * 54000 + 1 * 69000, SDS-PAGE Azotobacter chroococcum
69000
-
1 * 54000 + 1 * 69000, SDS-PAGE Azotobacter chroococcum
142000
-
gel filtration Azotobacter chroococcum

Organism

Organism UniProt Comment Textmining
Azotobacter chroococcum
-
SS2
-
Azotobacter chroococcum SS2
-
SS2
-

Purification (Commentary)

Purification (Comment) Organism
-
Azotobacter chroococcum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18.32
-
-
Azotobacter chroococcum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Hg + NADP+ + H+
-
Azotobacter chroococcum Hg2+ + NADPH
-
r
Hg + NADP+ + H+
-
Azotobacter chroococcum SS2 Hg2+ + NADPH
-
r
Hg2+ + NADPH
-
Azotobacter chroococcum Hg + NADP+ + H+
-
?
Hg2+ + NADPH
-
Azotobacter chroococcum SS2 Hg + NADP+ + H+
-
?

Subunits

Subunits Comment Organism
dimer 1 * 54000 + 1 * 69000, SDS-PAGE Azotobacter chroococcum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Azotobacter chroococcum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Azotobacter chroococcum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Azotobacter chroococcum