Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | required | Azotobacter chroococcum | |
2-mercaptoethanol | optimal concentration: 2 mM | Azotobacter chroococcum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 0.1 mM | Azotobacter chroococcum | |
Bi3+ | 0.1 mM, 40% inhibition | Azotobacter chroococcum | |
Cd2+ | - |
Azotobacter chroococcum | |
CoCl2 | - |
Azotobacter chroococcum | |
Cu2+ | 0.1 mM, 44% inhibition | Azotobacter chroococcum | |
HgCl2 | activity is inhibited by an excess of HgCl2 | Azotobacter chroococcum | |
NaN3 | - |
Azotobacter chroococcum | |
NEM | 2 mM, 60% inhibition | Azotobacter chroococcum | |
Ni2+ | 0.1 mM, 62% inhibition | Azotobacter chroococcum | |
Pb2+ | 0.1 mM, 60% inhibition | Azotobacter chroococcum | |
Zn2+ | 0.1 mM, 60% inhibition | Azotobacter chroococcum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
Hg | - |
Azotobacter chroococcum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Azotobacter chroococcum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
1 * 54000 + 1 * 69000, SDS-PAGE | Azotobacter chroococcum |
69000 | - |
1 * 54000 + 1 * 69000, SDS-PAGE | Azotobacter chroococcum |
142000 | - |
gel filtration | Azotobacter chroococcum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter chroococcum | - |
SS2 | - |
Azotobacter chroococcum SS2 | - |
SS2 | - |
Purification (Comment) | Organism |
---|---|
- |
Azotobacter chroococcum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18.32 | - |
- |
Azotobacter chroococcum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Hg + NADP+ + H+ | - |
Azotobacter chroococcum | Hg2+ + NADPH | - |
r | |
Hg + NADP+ + H+ | - |
Azotobacter chroococcum SS2 | Hg2+ + NADPH | - |
r | |
Hg2+ + NADPH | - |
Azotobacter chroococcum | Hg + NADP+ + H+ | - |
? | |
Hg2+ + NADPH | - |
Azotobacter chroococcum SS2 | Hg + NADP+ + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 54000 + 1 * 69000, SDS-PAGE | Azotobacter chroococcum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Azotobacter chroococcum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Azotobacter chroococcum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Azotobacter chroococcum |