Protein Variants | Comment | Organism |
---|---|---|
E47A | mutation has almost no effect on the reaction with superoxide | Desulfarculus baarsii |
K48I | 20-fold lower second-order rate constant for the oxidation of the iron center by superoxide compared to wild-type enzyme, K48 may play a role in directing and stabilizing superoxide to the active site at center II | Desulfarculus baarsii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+/Fe3+ | - |
Desulfarculus baarsii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfarculus baarsii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and E47A and K48I mutant enzyme | Desulfarculus baarsii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
superoxide + reduced rubredoxin + 2 H+ = H2O2 + oxidized rubredoxin | very fast bimolecular reaction of iron center II with superoxide, followed by the formation of two successive intermediate species | Desulfarculus baarsii |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Desulfarculus baarsii |