Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Desulfarculus baarsii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+/Fe3+ | 1.97 iron atoms/subunit, enzyme contains two Fe-centers: center I contains a mononuclear ferric iron coordinated by four cysteines in distorted rubredoxin-type center, center II has a ferrous iron with square pyramidal coordination to four nitrogens from histidines as equatorial ligands and one sulfur from a cysteine as the axial ligand, the reduced form of center II can transfer 1 electron to superoxid anion very efficiently | Desulfarculus baarsii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14028 | - |
2 * 14028, ES-MS | Desulfarculus baarsii |
27000 | - |
gel filtration | Desulfarculus baarsii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfarculus baarsii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, gel filtration, anion exchange | Desulfarculus baarsii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced cytochrome c + superoxide + H+ | enzyme shows only very weak superoxide dismutase activity | Desulfarculus baarsii | cytochrome c + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 14028, ES-MS | Desulfarculus baarsii |
Synonyms | Comment | Organism |
---|---|---|
desulfoferrodoxin | - |
Desulfarculus baarsii |