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Literature summary for 1.15.1.1 extracted from
- Copper, zinc superoxide dismutase from Caragana jubata a thermostable enzyme that functions under a broad pH and temperature window (2016), Process Biochem., 51, 1434-1444 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain M15 | Caragana jubata |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | - |
Caragana jubata |  |
| KCN | - |
Caragana jubata | |
| additional information | the enzyme is resistant to denaturation by sodium dodecyl sulfate (SDS) and urea | Caragana jubata |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | Cj-Cu,Zn SOD is a hydrophilic protein with no transmembrane domains | Caragana jubata | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | a Cu,Zn SOD | Caragana jubata | |
| Zn2+ | a Cu,Zn SOD | Caragana jubata | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 superoxide + 2 H+ | Caragana jubata | - |
O2 + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caragana jubata | A5HIG4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain M15 by nickel affinity chromatography, dialysis, and ultrafiltration | Caragana jubata |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1547 | - |
purified recombinant enzyme, 0°C, pH 7.8 | Caragana jubata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 superoxide + 2 H+ | - |
Caragana jubata | O2 + H2O2 | - |
? | |
| additional information | enzyme activity assay method using riboflavin and nitroblue tetrazolium (NBT) reduction | Caragana jubata | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 17500, His-tagged enzyme, SDS-PAGE, 2 * 15160, sequence calculation | Caragana jubata |
| monomer | 1 * 17500, His-tagged enzyme, SDS-PAGE, 1 * 15160, sequence calculation | Caragana jubata |
| More | the enzyme exists in both monomeric and dimeric forms, the latter being more active. Circular dichroic spectroscopy analysis confirms the thermostable nature of Cj-Cu,Zn SOD, secondary structure analysis, overview | Caragana jubata |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cj-Cu, Zn SOD | - |
Caragana jubata |
| Cu, Zn superoxide dismutase | - |
Caragana jubata |
| SOD | - |
Caragana jubata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
4 | - |
Caragana jubata |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
80 | activity range between -10-80°C | Caragana jubata |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
circular dichroic spectroscopy analysis confirms the thermostable nature of Cj-Cu,Zn SOD. Thermal inactivation first-order kinetics | Caragana jubata |
| 80 | - |
recombinant enzyme SOD Cj-Cu,Zn SOD shows a residual SOD activity of 26.07% after being heated for 160 min | Caragana jubata |
| 121 | - |
Cj-Cu,Zn SOD retains 50% of the maximum activity after autoclaving, autoclaved Ca-Cu,Zn SOD exhibits 40% of maximum activity of unautoclaved enzyme at 20°C and 30°C. Thermal inactivation kinetics of Ca-Cu,Zn SOD, overview | Caragana jubata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Caragana jubata |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | activity range | Caragana jubata |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 10 | purified recombinant His-tagged enzyme, stable for 2 and 24 h of incubation,after which it retains over 80% activity, 2030% decrease in activity is observed at pH 10.0 | Caragana jubata |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Caragana jubata | sequence calculation | - |
5.82 |
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