Application | Comment | Organism |
---|---|---|
medicine | SOD3 has the potential as a therapeutic drug for various diseases | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene SOD3, cloning of EC-SOD and recombinant expression of wild-type and truncated mutant FLAG-tagged hEC-SOD enzymes in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method, both full length and truncated hEC-SOD proteins are enzymatically active | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a catalytically active truncated (residues 19-240) mutant form of human EC-SOD (hEC-SOD) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyldithiocarbamate | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | required, enzyme-bound | Homo sapiens | |
additional information | added Zn2+ and Cu2+ do not affect the enzyme activity or structure | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 superoxide + 2 H+ | Homo sapiens | - |
O2 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P08294 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | recombinant hEC-SOD expressed in Sf9 insect cells is glycosylated at Asn107. The glycosylated form of hEC-SOD is soluble, whereas the unglycosylated form is not, but it can be solubilized by increasing the pH and ionic strength of the solution | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and truncated mutant FLAG-tagged hEC-SOD enzymes from Spodoptera frugiperda Sf9 cells by affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 superoxide + 2 H+ | - |
Homo sapiens | O2 + H2O2 | - |
? | |
additional information | enzyme activity detection by water-soluble tetrazolium (WST-1) assay. This assay is based on the detection of a water-soluble formazan dye that is formed upon reduction of water-soluble tetrazolium salt, WST-1, by the superoxide anion | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 33000, recombinant enzyme, SDS-PAGE | Homo sapiens |
monomer | 1 * 33000, recombinant enzyme, SDS-PAGE | Homo sapiens |
More | hEC-SOD is present both as a monomer (33 kDa) and a dimer (66 kDa), enzyme secondary structure determination by circular dichroism, recombinant hEC-SOD purified from Sf9 insect cells is mainly composed of beta-sheet structures | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
EC-SOD | - |
Homo sapiens |
extracellular superoxide dismutase | - |
Homo sapiens |
hEC-SOD | - |
Homo sapiens |
SOD3 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 9 | assay at | Homo sapiens |