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Literature summary for 1.15.1.1 extracted from
- Cloning, characterization and expression pattern analysis of a cytosolic copper/zinc superoxide dismutase (SaCSD1) in a highly salt tolerant mangrove (Sonneratia alba) (2015), Int. J. Mol. Sci., 17, E4 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| chloroform | the enzyme activity increases by about 20% in glycerol or chloroform, glycerol and chloroform may supply a hydrophobic environment that is conducive to better contact with the substrate | Sonneratia alba |  |
| glycerol | the enzyme activity increases by about 20% in glycerol or chloroform, glycerol and chloroform may supply a hydrophobic environment that is conducive to better contact with the substrate | Sonneratia alba | |
| additional information | enzyme SaCSD1 is degraded rapidly and completely after enzymatic treatment with pepsin. No full-length protein is detectable even after 0.5 min of digestion | Sonneratia alba |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene CSD1, DNA and amino acid sequence determination and analysis, sequence comparisons, real-time quantitative PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta-gami | Sonneratia alba |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Sonneratia alba | |
| H2O2 | - |
Sonneratia alba | |
| additional information | the SaCSD1 protein is very susceptive to pepsin digestion | Sonneratia alba | |
| NaCl | activity of SaCSD1 decreases with the increase of salt concentration | Sonneratia alba | |
| phenol | - |
Sonneratia alba | |
| SDS | - |
Sonneratia alba | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Sonneratia alba | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | a Cu/Zn-SOD | Sonneratia alba | |
| Zn2+ | a Cu/Zn-SOD | Sonneratia alba | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 superoxide + 2 H+ | Sonneratia alba | - |
O2 + H2O2 | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| chloroform | the enzyme activity increases by about 20% in glycerol or chloroform | Sonneratia alba |
| DMSO | 90% residual activity in DMSO | Sonneratia alba |
| Glycerol | the enzyme activity increases by about 20% in glycerol or chloroform | Sonneratia alba |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sonneratia alba | A0A0U1X631 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | the SaCSD1 protein is very susceptive to pepsin digestion | Sonneratia alba |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme 3.2fold from Escherichia coli strain Rosetta-gami by nickel affinity chromatography | Sonneratia alba |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Sonneratia alba | - |
| fruit | - |
Sonneratia alba | - |
| leaf | - |
Sonneratia alba | - |
| additional information | enzyme expression pattern analysis | Sonneratia alba | - |
| stem | - |
Sonneratia alba | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2200 | - |
purified recombinant His-tagged enzyme, pH 6.0, 25°C | Sonneratia alba |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 superoxide + 2 H+ | - |
Sonneratia alba | O2 + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 15320, SaCSD1, sequence calculation | Sonneratia alba |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| copper/zinc superoxide dismutase | - |
Sonneratia alba |
| SaCSD1 | - |
Sonneratia alba |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | broad optimum | Sonneratia alba |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 55 | high activity range | Sonneratia alba |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 75 | purified recombinant His-tagged enzyme, no significant change in enzyme activity at 25-45°C for up to 1 h, at 75°C, 60% of enzyme activity is retained after 1 h of treatment, suggesting that the SaCSD1 is relatively thermostable | Sonneratia alba |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Sonneratia alba |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | high activity range | Sonneratia alba |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | purified recombinant His-tagged enzyme, stable at | Sonneratia alba |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Sonneratia alba | sequence calculation | - |
5.78 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Sonneratia alba | under 0.25 M and 0.5 M salt stress, the expression of SaCSD1 is downregulated in roots, but upregulated in leaves | down |
| Sonneratia alba | under 0.25 M and 0.5 M salt stress, the expression of SaCSD1 is downregulated in roots, but upregulated in leaves | up |
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