Cloned (Comment) | Organism |
---|---|
gene sodA, expression of SodA in and complementation of enzyme-deficient Escherichia coli strain PN134. Expressing YeSodA confers resistance to paraquat to the Escherichia coli strain | Yersinia enterocolitica |
gene sodB, expression of SodB in and complementation of enzyme-deficient Escherichia coli strain PN134. Expressing YeSodB confers resistance to paraquat to the Escherichia coli strain | Yersinia enterocolitica |
gene sodC, SodC cannot be expressed in Escherichia coli strain BL21(DE3) | Yersinia enterocolitica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chloroform:ethanol solution | the Mn- and Fe-SODs of Yérsinia enterocolitica are inhibited by chloroform:ethanol solution; the Mn- and Fe-SODs of Yérsinia enterocolitica are inhibited by chloroform:ethanol solution; the Mn- and Fe-SODs of Yérsinia enterocolitica are inhibited by chloroform:ethanol solution | Yersinia enterocolitica | |
diethyldithiocarbamate | inhibits SodC specifically | Yersinia enterocolitica | |
additional information | no inhibition by diethyldithiocarbamate; no inhibition by diethyldithiocarbamate | Yersinia enterocolitica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Yersinia enterocolitica | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | a Mn-Fe-SOD | Yersinia enterocolitica | |
Mn2+ | a Mn-Fe-SOD | Yersinia enterocolitica | |
additional information | binding ligands: His27, His74, Asp157 and His161 in SodB | Yersinia enterocolitica | |
additional information | binding ligands: His27, His82, Asp169 and His173 in SodA | Yersinia enterocolitica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
SodB, gel filtration | Yersinia enterocolitica |
23000 | - |
4 * 23000, recombinant SodA, SDS-PAGE | Yersinia enterocolitica |
82000 | - |
SodA, gel filtration | Yersinia enterocolitica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yersinia enterocolitica | A9U908 | gene sodA | - |
Yersinia enterocolitica | J7H7U2 | gene sodC | - |
Yersinia enterocolitica | J7HET3 | genes sodA, sodB, and sodC | - |
Yersinia enterocolitica IP27366 | A9U908 | gene sodA | - |
Yersinia enterocolitica IP27366 | J7H7U2 | gene sodC | - |
Yersinia enterocolitica IP27366 | J7HET3 | genes sodA, sodB, and sodC | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12800 | - |
purified recombinant SodA, 4°C, pH 6.0 | Yersinia enterocolitica |
24400 | - |
purified recombinant SodB, 4°C, pH 4.0 | Yersinia enterocolitica |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 21000, recombinant SodB, SDS-PAGE | Yersinia enterocolitica |
More | secondary structure analysis using circular dichroism, overview. At pH 7.0 and 28°C, SodA contains 29% alpha-helix and 16% beta-sheets. The elements of secondary structures are more sensitive to pH than to temperature | Yersinia enterocolitica |
More | secondary structure analysis using circular dichroism, overview. At pH 7.0 and 28°C, SodB contains 44% alpha-helix and 13% beta-sheets. The elements of secondary structures are more sensitive to pH than to temperature | Yersinia enterocolitica |
tetramer | 4 * 23000, recombinant SodA, SDS-PAGE | Yersinia enterocolitica |
Synonyms | Comment | Organism |
---|---|---|
SODA | - |
Yersinia enterocolitica |
SodB | - |
Yersinia enterocolitica |
SodC | ambiguous | Yersinia enterocolitica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
- |
Yersinia enterocolitica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
SodB | Yersinia enterocolitica |
6 | - |
SodA | Yersinia enterocolitica |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Yersinia enterocolitica | SodB, isoelectric focusing | - |
5.8 |
Yersinia enterocolitica | SodA, isoelectric focusing | - |
6.2 |
General Information | Comment | Organism |
---|---|---|
additional information | secondary structure analysis using circular dichroism, overview | Yersinia enterocolitica |