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Literature summary for 1.15.1.1 extracted from

  • Mahanty, S.; Kaul, T.; Pandey, P.; Reddy, R.A.; Mallikarjuna, G.; Reddy, C.S.; Sopory, S.K.; Reddy, M.K.
    Biochemical and molecular analyses of copper-zinc superoxide dismutase from a C4 plant Pennisetum glaucum reveals an adaptive role in response to oxidative stress (2012), Gene, 505, 309-317.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Cenchrus americanus

Inhibitors

Inhibitors Comment Organism Structure
H2O2 50% inhibition at 4.3 mM Cenchrus americanus
additional information no inhibition by 10 mM NaN3 Cenchrus americanus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Cenchrus americanus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ a CuZn-SOD Cenchrus americanus
Zn2+ a CuZn-SOD Cenchrus americanus

Organism

Organism UniProt Comment Textmining
Cenchrus americanus A4ZYP8
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to near homogeneity Cenchrus americanus

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Cenchrus americanus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information SOD activity is assayed based on its ability to compete with nitroblue-tetrazolium for superoxide anions generated by the xanthine-xanthine oxidase system, which in turn results in the inhibition of reduction of nitroblue-tetrazolium Cenchrus americanus ?
-
?

Subunits

Subunits Comment Organism
More homology structure modeling of PgCuZnSOD, overview Cenchrus americanus

Synonyms

Synonyms Comment Organism
copper-zinc superoxide dismutase
-
Cenchrus americanus
CuZnSOD
-
Cenchrus americanus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
44.5
-
purified recombinant His-tagged enzyme, 60 min, loss of 50% activity Cenchrus americanus
70
-
purified recombinant His-tagged enzyme, 60 min, loss of 96% activity Cenchrus americanus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
Cenchrus americanus

pH Range

pH Minimum pH Maximum Comment Organism
7 10 maximal activity at pH 7.6, 50% of miximal activity at pH 8.5, inactivation above pH 10.0 Cenchrus americanus

pI Value

Organism Comment pI Value Maximum pI Value
Cenchrus americanus isoelectric focusing
-
5.76

Expression

Organism Comment Expression
Cenchrus americanus in Pennisetum seedlings, abiotic stress-induced PgCuZnSOD transcript upregulation directly correlates to high protein and activity induction. PgCuZnSOD mRNA levels gradually increase to several folds on exposure of Pennisetum seedlings corresponding to gradual increase in the time period of different stress treatments i.e. dehydration, methyl viologen, NaCl and high temperature (48°C) with an exception in cold stress (4°C) where the transcript initially increased (0-12 h time-point) in comparison to control and later decreased (24 h time-point) with regards to initial rise up

General Information

General Information Comment Organism
additional information overexpression of PgCuZnSOD confers comparatively enhanced tolerance to methyl viologen induced oxidative stress in bacteria. Homology structure modeling of PgCuZnSOD, overview Cenchrus americanus
physiological function PgCuZnSOD plays a functional role in conferring oxidative stress tolerance to prokaryotic system Cenchrus americanus