Cloned (Comment) | Organism |
---|---|
gene sodA, DNA and amino acid sequence determination and analysis, sequence comparison | Exiguobacterium sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | MnSOD, the Mn ion is the only metal cofactor, 0.57 atom per polypeptide chain | Exiguobacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22532 | - |
2 * 23500, SDS-PAGE, 2 * 22532, sequence calculation | Exiguobacterium sp. |
23500 | - |
2 * 23500, SDS-PAGE, 2 * 22532, sequence calculation | Exiguobacterium sp. |
50000 | - |
gel filtration | Exiguobacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Exiguobacterium sp. | - |
collected from a tepid spring, 41°C, pH 7.9, in Oguni-tyo, Kumamoto, Japan, gene sodA | - |
Exiguobacterium sp. OS-77 | - |
collected from a tepid spring, 41°C, pH 7.9, in Oguni-tyo, Kumamoto, Japan, gene sodA | - |
Purification (Comment) | Organism |
---|---|
native enzyme 50fold from strain OS-77 by hydrophobic interaction and anion exchange chromatography, followed by gel filtration | Exiguobacterium sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | growth temperature range of the strain OS-77 is between 5°C and 40°C with its optimum growth temperature of 20°C, the growth pH range of the strain OS-77 is between pH 6.0 and pH 10.0 with optimum growth at pH 8.0, strain OS-77 shows salinity tolerance up to 7.5% w/v NaCl solution | Exiguobacterium sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10500 | - |
purified native enzyme, pH 7.8, 25°C | Exiguobacterium sp. |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 23500, SDS-PAGE, 2 * 22532, sequence calculation | Exiguobacterium sp. |
Synonyms | Comment | Organism |
---|---|---|
MnSOD | - |
Exiguobacterium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | - |
- |
Exiguobacterium sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 25 | optimum activity at 5°C, 80% of maximal activity at 25°C | Exiguobacterium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
two protein denaturation peaks at 65°C and 84°C by differential scanning calorimetry | Exiguobacterium sp. |
45 | - |
stable up to | Exiguobacterium sp. |
60 | - |
21.2 min, loss of 50% activity | Exiguobacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Exiguobacterium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | 70% of maximal activity within this range | Exiguobacterium sp. |
General Information | Comment | Organism |
---|---|---|
additional information | residues involved in the active site are H28, Y36, H82, Q149, D164, and H168, which are completely conserved in the EgMnSOD | Exiguobacterium sp. |
physiological function | the enzyme may play an important role as an antioxidant in a wide temperature range | Exiguobacterium sp. |