General Stability | Organism |
---|---|
activity of the SOD remains intact both in crude and purified forms after autoclaving at 6-20 bars up to 10 min and microwaving at a frequency of 2450 MHz or million cycles per second for 1-3 min | Curcuma longa |
polyvinylchloride stabilizes the enzyme | Curcuma longa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | inhibition is reversible by Cu and Zn | Curcuma longa | |
H2O2 | - |
Curcuma longa | |
KCN | - |
Curcuma longa | |
Sodium diethyldithiocarbamate | complete inhibition above 0.1 mM | Curcuma longa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Curcuma longa | 9507 | - |
cytoplasm | - |
Curcuma longa | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu | a Cu-Zn SOD, activates | Curcuma longa | |
Zn | a Cu-Zn SOD | Curcuma longa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15000 | - |
2 * 15000, SDS-PAGE | Curcuma longa |
30800 | - |
native PAGE | Curcuma longa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Curcuma longa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Curcuma longa | - |
Storage Stability | Organism |
---|---|
4°C, purified enzyme, stable for at least 18 months | Curcuma longa |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 15000, SDS-PAGE | Curcuma longa |
Synonyms | Comment | Organism |
---|---|---|
Cu-Zn SOD | - |
Curcuma longa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified enzyme, completely stable after 20 min, after 1 h, 98% remaining activity of the leaf enzyme, 88% of the rhizome enzyme | Curcuma longa |
60 | - |
purified enzyme, completely stable after 20 min, after 1 h, 90% remaining activity of the leaf enzyme, 70% of the rhizome enzyme | Curcuma longa |
80 | - |
purified enzyme, completely stable after 20 min, after 1 h, 80% remaining activity of the leaf enzyme, 52% of the rhizome enzyme | Curcuma longa |
100 | - |
purified enzyme, loss of 5% activity after 10 min boiling for the chloroplast enzyme and 35% for the cytosolic enzyme, after 1 h, 70% remaining activity, of the leaf enzyme, 20% of the rhizome enzyme | Curcuma longa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | assay at | Curcuma longa |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.04 | - |
- |
Curcuma longa | Sodium diethyldithiocarbamate |